Mandache E, Moldoveanu E, Moţa G, Moraru I, Gheţie V
J Immunol Methods. 1980;35(1-2):33-41. doi: 10.1016/0022-1759(80)90148-9.
Multivalent hybrid antibody complexes with dual specificity were prepared by combining rabbit antibody with anti-mouse immunoglobulin (mIg) and anti-peroxidase (HRP) specificity using protein A of Staphylococcus aureus. The presence of two antibody molecules with anti-mIg and anti-HRP specificity in a single molecule of hybrid complex was demonstrated by their abilities to produce hemagglutination with both HRP-coated and mIg-coated sheep red cells, to give a reaction of complete identity with mIg and HRP and to allow mIg bearing lymphocytes to form rosettes with HRP-coated sheep red blood cells. Electron microscopy of mouse lymphocytes and thymocytes (previously coated with mIg anti-Thy-1 antibody) treated with hybrid antibody complex and HRP showed strong and specific staining of the cell membrane of both cell types. The hybrid antibody complex containing anti-HRP antibody is a valuable reagent for determining various antigenic markers on cell membranes by electron microscopy.
通过使用金黄色葡萄球菌的蛋白A将具有抗小鼠免疫球蛋白（mIg）和抗过氧化物酶（HRP）特异性的兔抗体结合，制备了具有双重特异性的多价杂交抗体复合物。通过杂交复合物的单个分子中存在两种具有抗mIg和抗HRP特异性的抗体分子，这表现为它们能够与HRP包被的和mIg包被的绵羊红细胞产生血凝反应，与mIg和HRP产生完全相同的反应，并使携带mIg的淋巴细胞与HRP包被的绵羊红细胞形成花环。用杂交抗体复合物和HRP处理的小鼠淋巴细胞和胸腺细胞（先前用抗Thy-1抗体包被mIg）的电子显微镜检查显示，两种细胞类型的细胞膜均有强烈且特异性的染色。含有抗HRP抗体的杂交抗体复合物是通过电子显微镜测定细胞膜上各种抗原标志物的有价值的试剂。
