Kreavich M E, Meyer J, Waterhouse J P
J Oral Pathol. 1981 Feb;10(1):22-31. doi: 10.1111/j.1600-0714.1981.tb01244.x.
Six weanling male Sprague Dawley rats were fed a diet containing 0.4 ppm Zn and seven were fed an identical diet except for 40 ppm Zn. After 4 weeks, specimens of buccal mucosa in the region facing the molar teeth were removed. Paraffin sections, cut at 6 micron, were stained with toluidine blue, and tracings made of five sections per animal, spaced no less than 60 micron apart. Counts of mast cells of five sections length of section were made in a superficial zone of the lamina propria of 50 micron width and a deeper zone of 250 micron width. The average number of mast cells, per mm in the subepithelial zone of the experimental animals was 15.4, the range 9.2-33.1. The control average was 4.0; the range was 2.9-5.3. No increase was found in the deeper zone. The epithelium was parakeratotic and its thickness was increased two-fold. In the peripheral portion of the section, cellular and keratin layers were evenly increased in thickness, but in the central portion a disproportionate, nearly four-fold increase occurred in the keratin layer and a lesser increase in the cellular layer.
六只断乳雄性斯普拉格-道利大鼠喂食含0.4 ppm锌的饲料，七只喂食除锌含量为40 ppm外其他相同的饲料。4周后，取下磨牙对面区域的颊黏膜标本。将切成6微米厚的石蜡切片用甲苯胺蓝染色，每只动物选取五张切片进行描图，切片间距不少于60微米。在宽度为50微米的固有层浅表层和250微米宽的较深层对五张切片长度内的肥大细胞进行计数。实验动物上皮下区域每毫米肥大细胞的平均数量为15.4，范围为9.2至33.1。对照组的平均值为4.0；范围为2.9至5.3。在较深层未发现增加。上皮为不全角化，其厚度增加了两倍。在切片的周边部分，细胞层和角质层厚度均匀增加，但在中央部分，角质层增加不成比例，几乎增加了四倍，细胞层增加较少。
