Irons R D, Schenk E A, Lee J C
Arch Pathol Lab Med. 1977 Jun;101(6):298-301.
Three previously described histochemical methods for the localization of copper were examined for applicability in the semiquantitative evaluation copper levels in human tissue. Liver tissue obtained at autopsy was stained using dimethylaminobenzylidene rhodanine, rubeanic acid, and diethyldithiocarbamate procedures. The effects of method and time of fixation, staining, incubation time, and variations in procedures were evaluated. Copper levels in tissues examined were determined using atomic absorption spectroscopy. Each staining method was evaluated for sensitivity, reproducibility, and linearity. The rhodanine method was found to produce the most reproducible results, and a linear relationship between microscopical evaluation of the stain and actual tissue copper levels was observed. It was concluded that the rhodanine copper method is applicable for the semi-quantitative evaluation of tissue copper and provides a satisfactory screening method for the identification of abnormal tissue copper levels.
研究了三种先前描述的用于铜定位的组织化学方法在半定量评估人体组织中铜水平方面的适用性。对尸检获得的肝组织采用二甲基氨基苄叉罗丹宁、硫氰酸和二乙基二硫代氨基甲酸盐程序进行染色。评估了固定方法和时间、染色、孵育时间以及程序变化的影响。使用原子吸收光谱法测定所检查组织中的铜水平。对每种染色方法的灵敏度、重现性和线性进行了评估。发现罗丹宁法产生的结果重现性最佳，并且观察到染色的显微镜评估与实际组织铜水平之间存在线性关系。得出结论，罗丹宁铜法适用于组织铜的半定量评估，并为识别异常组织铜水平提供了一种令人满意的筛查方法。
