Seki S, Oda T, Ohashi M
Biochim Biophys Acta. 1980 Dec 11;610(2):413-20. doi: 10.1016/0005-2787(80)90022-2.
Aphidicolin clearly discriminated replicative DNA synthesis from unscheduled DNA synthesis. Aphidicolin inhibited replicative DNA synthesis in permeable mouse ascites sarcoma cells. The mode of inhibition of aphidicolin was a mixed type with respect to deoxycytidine triphosphate but was non-competitive with respect to the other three deoxynucleoside triphosphates. Aphidicolin did not affect the activity of unscheduled DNA synthesis in either bleomycin-treated permeable sarcoma cells or isolated rat liver nuclei. Considering the difference in sensitivity of DNA polymerase alpha and beta to aphidicolin, and other related information reported previously, the results are compatible with the idea that DNA polymerase alpha is involved in replicative DNA synthesis and DNA polymerase beta in unscheduled DNA synthesis in the present systems.
阿非迪霉素能明确区分复制性DNA合成与非程序性DNA合成。阿非迪霉素抑制可渗透的小鼠腹水肉瘤细胞中的复制性DNA合成。就脱氧胞苷三磷酸而言，阿非迪霉素的抑制模式为混合型，但就其他三种脱氧核苷三磷酸而言则为非竞争性。阿非迪霉素对博来霉素处理的可渗透肉瘤细胞或分离的大鼠肝细胞核中的非程序性DNA合成活性均无影响。考虑到DNA聚合酶α和β对阿非迪霉素敏感性的差异以及先前报道的其他相关信息，这些结果与以下观点相符：在本系统中，DNA聚合酶α参与复制性DNA合成，而DNA聚合酶β参与非程序性DNA合成。
