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黑腹果蝇中一个基因座大小的分子限制

Molecular limits on the size of a genetic locus in Drosophila melanogaster.

作者信息

McGinnis W, Farrell J, Beckendorf S K

出版信息

Proc Natl Acad Sci U S A. 1980 Dec;77(12):7367-71. doi: 10.1073/pnas.77.12.7367.

DOI:10.1073/pnas.77.12.7367
PMID:6784122
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC350504/
Abstract

This report places outer limits on the size of the DNA region required for expression of a Drosophila gene. This region, termed the unit of expression, includes not only the structural gene but also any cis-acting sequences that modulate its activity. The locus we have chosen, Sgs-4, codes for one of the glue proteins secreted by larval Drosophila salivary glands. Cytological deficiencies have been identified that eliminate sequences on one side or the other of Sgs-4 without affecting its expression. The ends of these deficiencies have been localized accurately with respect to restriction endonuclease sites in and near the locus. These endpoints limit the Sgs-4 structural gene and essential flanking sequences to a 16- to 19-kilobase region of the X chromosome. The results also show that there is no DNA sequence rearrangement in the Sgs-4 region during development of either the polytene larval salivary glands or adult flies.

摘要

本报告确定了果蝇基因表达所需DNA区域大小的外部界限。该区域，即表达单位，不仅包括结构基因，还包括任何调节其活性的顺式作用序列。我们选择的基因座Sgs-4编码果蝇幼虫唾液腺分泌的一种胶蛋白。已鉴定出细胞学缺失，这些缺失消除了Sgs-4一侧或另一侧的序列，但不影响其表达。这些缺失的末端已相对于该基因座及其附近的限制性内切酶位点准确定位。这些端点将Sgs-4结构基因和必需的侧翼序列限制在X染色体上16至19千碱基的区域内。结果还表明，在多线幼虫唾液腺或成年果蝇发育过程中，Sgs-4区域没有DNA序列重排。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd7f/350504/a138bf6e04fa/pnas00499-0432-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd7f/350504/f33f5f05c634/pnas00499-0430-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd7f/350504/d4314e4274d3/pnas00499-0430-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd7f/350504/51fea7257bc0/pnas00499-0431-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd7f/350504/03be76c019e4/pnas00499-0431-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd7f/350504/27283186d815/pnas00499-0431-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd7f/350504/c154e6e9a434/pnas00499-0432-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd7f/350504/a138bf6e04fa/pnas00499-0432-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd7f/350504/f33f5f05c634/pnas00499-0430-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd7f/350504/d4314e4274d3/pnas00499-0430-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd7f/350504/51fea7257bc0/pnas00499-0431-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd7f/350504/03be76c019e4/pnas00499-0431-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd7f/350504/27283186d815/pnas00499-0431-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd7f/350504/c154e6e9a434/pnas00499-0432-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd7f/350504/a138bf6e04fa/pnas00499-0432-b.jpg

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本文引用的文献

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Nonessential Sequences, Genes, and the Polytene Chromosome Bands of DROSOPHILA MELANOGASTER.非必需序列、基因和黑腹果蝇的多线染色体带。
Genetics. 1978 Apr;88(4):723-42. doi: 10.1093/genetics/88.4.723.
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Molecular analysis of a gene in a developmentally regulated puff of Drosophila melanogaster.黑腹果蝇发育调控的唾腺染色体疏松区中一个基因的分子分析。
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7362-6. doi: 10.1073/pnas.77.12.7362.
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gamma-beta-Thalassaemia studies showing that deletion of the gamma- and delta-genes influences beta-globin gene expression in man.
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Genetic analysis of the larval secretion gene Sgs-4 and its regulatory chromosome sites in Drosophila melanogaster.黑腹果蝇幼虫分泌基因Sgs-4及其调控染色体位点的遗传分析。
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Molecular analysis of a gene in a developmentally regulated puff of Drosophila melanogaster.黑腹果蝇发育调控的唾腺染色体疏松区中一个基因的分子分析。
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7362-6. doi: 10.1073/pnas.77.12.7362.
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Genetic analysis of chromomere 3D4 in Drosophila melanogaster. II. Regulatory sites for the dunce gene.黑腹果蝇3D4染色粒的遗传分析。II. 笨蛋基因的调控位点。
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DNA sequence changes in an upstream DNase I-hypersensitive region are correlated with reduced gene expression.上游DNA酶I超敏反应区域的DNA序列变化与基因表达降低相关。
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A cytological approach to the ordering of events in gene activation using the Sgs-4 locus of Drosophila melanogaster.利用黑腹果蝇的Sgs-4基因座，采用细胞学方法对基因激活过程中的事件进行排序。
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Adv Genet. 1968;14:133-69.
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Cell. 1976 Nov;9(3):365-73. doi: 10.1016/0092-8674(76)90081-7.
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