Verkleij A J, de Kruijff B, van Echteld C J, Gerritsen W J, Mombers C, Noordam P C, Leunissen-Bijvelt J, de Gier J
Acta Histochem Suppl. 1981;23:145-9.
A new type of lipid organization is observed in mixtures of phosphatidylcholine with cardiolipin in the presence of Ca++, monoglucosyldiglyceride and phosphatidylethanolamine (in the presence of cholesterol). This phase is characterized by an isotropic 31P NMR signal and is visualized by freeze fracturing as particles and pits on the fracture faces of the lipid bilayer. As the most favourable model for this phase we propose the inverted micelle sandwiched in between the two monolayers of the lipid bilayer. It will be shown that such particles and pits appear on the fusion site during fusion of unilamellar vesicles of an equimolar mixture of phosphatidylcholine and cardiolipin in the presence of Ca++.
在钙离子、单葡萄糖甘油二酯和磷脂酰乙醇胺存在的情况下（在胆固醇存在时），观察到磷脂酰胆碱与心磷脂混合物中存在一种新型脂质组织。该相的特征是具有各向同性的31P NMR信号，通过冷冻断裂可在脂质双分子层的断裂面上观察到颗粒和凹坑。作为该相最有利的模型，我们提出了夹在脂质双分子层两个单分子层之间的反胶束。结果将表明，在钙离子存在的情况下，磷脂酰胆碱和心磷脂等摩尔混合物的单层囊泡融合过程中，融合位点会出现此类颗粒和凹坑。
