木瓜蛋白酶的低温酶学：与酯底物的反应机制 - Suppr

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Cryoenzymology of papain: reaction mechanism with an ester substrate.

作者信息

Angelides K J, Fink A L

出版信息

Biochemistry. 1978 Jun 27;17(13):2659-68. doi: 10.1021/bi00606a032.

DOI:10.1021/bi00606a032

PMID:678536

Abstract

摘要

相似文献

Cryoenzymology of papain: reaction mechanism with an ester substrate.木瓜蛋白酶的低温酶学：与酯底物的反应机制

Biochemistry. 1978 Jun 27;17(13):2659-68. doi: 10.1021/bi00606a032.

The trypsin-catalyzed hydrolysis of N alpha-benzyloxycarbonyl-L-lysine p-nitrophenyl ester in dimethylsulfoxide at sub-zero temperatures.胰蛋白酶在零下温度的二甲基亚砜中催化水解Nα-苄氧羰基-L-赖氨酸对硝基苯酯。

J Biol Chem. 1974 Aug 25;249(16):5027-32.

Papain-catalyzed reactions at subzero temperatures.木瓜蛋白酶在零下温度下催化的反应。

Biochemistry. 1976 Nov 30;15(24):5287-93. doi: 10.1021/bi00669a014.

Comparison of the kinetics and mechanism of the papain-catalyzed hydrolysis of esters and thiono esters.木瓜蛋白酶催化酯类和硫代酯水解的动力学及机理比较

Biochemistry. 1985 Nov 19;24(24):6808-18. doi: 10.1021/bi00345a012.

Conformational change associated with the acylation of alpha-chymotrypsin by N-acetyl-L-phenylalanine methyl ester.与N-乙酰-L-苯丙氨酸甲酯对α-胰凝乳蛋白酶进行酰化作用相关的构象变化。

J Biol Chem. 1974 Oct 10;249(19):6087-9.

Comparison of the kinetics of the papain-catalyzed hydrolysis of glycine- and alanine-based esters and thiono esters.木瓜蛋白酶催化甘氨酸和丙氨酸基酯及硫代酯水解动力学的比较。

Biochemistry. 1988 Jan 12;27(1):264-8. doi: 10.1021/bi00401a040.

Cryoenzymology: the study of enzyme catalysis at subzero temperatures.

Methods Enzymol. 1979;63:336-70. doi: 10.1016/0076-6879(79)63015-x.

Influence of Me2SO and incubation time on papain activity studied using fluorogenic substrates.

Acta Biochim Pol. 2001;48(4):995-1002.

Kinetics of the hydrolysis of N-benzoyl-L-serine methyl ester catalysed by bromelain and by papain. Analysis of modifier mechanisms by lattice nomography, computational methods of parameter evaluation for substrate-activated catalyses and consequences of postulated non-productive binding in bromelain- and papain-catalysed hydrolyses.菠萝蛋白酶和木瓜蛋白酶催化N-苯甲酰-L-丝氨酸甲酯水解的动力学。通过晶格列线图分析修饰机制、底物活化催化的参数评估计算方法以及菠萝蛋白酶和木瓜蛋白酶催化水解中假定的非生产性结合的后果。

Biochem J. 1974 Aug;141(2):365-381. doi: 10.1042/bj1410365.

On the enhanced catalytic activity of papain towards amide substrates.关于木瓜蛋白酶对酰胺底物的增强催化活性。

Acta Biochim Biophys Acad Sci Hung. 1977;12(3):223-30.

引用本文的文献

Differences in the interaction of the catalytic groups of the active centres of actinidin and papain. Rapid purification of fully active actinidin by covalent chromatography and characterization of its active centre by use of two-protonic-state reactivity probes.猕猴桃蛋白酶和木瓜蛋白酶活性中心催化基团相互作用的差异。通过共价色谱法快速纯化完全活性的猕猴桃蛋白酶，并使用双质子态反应性探针表征其活性中心。

Biochem J. 1981 Sep 1;197(3):739-46. doi: 10.1042/bj1970739.

Evidence for a two-state transition in papain that may have no close analogue in ficin. Differences in the disposition of cationic sites and hydrophobic binding areas in the active centres of papain and ficin.木瓜蛋白酶中可能在无花果蛋白酶中没有类似情况的双态转变的证据。木瓜蛋白酶和无花果蛋白酶活性中心中阳离子位点和疏水结合区域分布的差异。

Biochem J. 1980 Dec 1;191(3):707-18. doi: 10.1042/bj1910707.

Current problems in mechanistic studies of serine and cysteine proteinases.丝氨酸蛋白酶和半胱氨酸蛋白酶机制研究中的当前问题。

Biochem J. 1982 Oct 1;207(1):1-10. doi: 10.1042/bj2070001.

Characterization of papaya peptidase A as a cysteine proteinase of Carica papaya L. with active-centre properties that differ from those of papain by using 2,2'-dipyridyl disulphide and 4-chloro-7-nitrobenzofurazan as reactivity probes. Use of two-protonic-state electrophiles in the identification of catalytic-site thiol groups.以2,2'-二吡啶二硫化物和4-氯-7-硝基苯并呋喃为反应探针，表征番木瓜蛋白酶A为番木瓜（Carica papaya L.）的一种半胱氨酸蛋白酶，其活性中心特性与木瓜蛋白酶不同。使用双质子态亲电试剂鉴定催化位点的巯基。

Biochem J. 1982 Jul 1;205(1):205-11. doi: 10.1042/bj2050205.

Natural structural variation in enzymes as a tool in the study of mechanism exemplified by a comparison of the catalytic-site structure and characteristics of cathepsin B and papain. pH-dependent kinetics of the reactions of cathepsin B from bovine spleen and from rat liver with a thiol-specific two-protonic-state probe (2,2'-dipyridyl disulphide) and with a specific synthetic substrate (N-alpha-benzyloxycarbonyl-L-arginyl-L-arginine 2-naphthylamide).酶的天然结构变异作为研究机制的工具：以组织蛋白酶B和木瓜蛋白酶催化位点结构及特性的比较为例。牛脾脏和大鼠肝脏组织蛋白酶B与硫醇特异性双质子态探针（2,2'-二吡啶二硫化物）以及特异性合成底物（N-α-苄氧羰基-L-精氨酰-L-精氨酸2-萘酰胺）反应的pH依赖性动力学。

Biochem J. 1984 Sep 15;222(3):805-14. doi: 10.1042/bj2220805.

Effects of conformational selectivity and of overlapping kinetically influential ionizations on the characteristics of pH-dependent enzyme kinetics. Implications of free-enzyme pKa variability in reactions of papain for its catalytic mechanism.构象选择性和重叠动力学影响电离对pH依赖性酶动力学特征的影响。木瓜蛋白酶反应中游离酶pKa变异性对其催化机制的影响。

Biochem J. 1983 Jun 1;211(3):701-8. doi: 10.1042/bj2110701.

Kinetic solvent isotope effects on the deacylation of specific acyl-papains. Proton inventory studies on the papain-catalysed hydrolyses of specific ester substrates: analysis of possible transition state structures.动力学溶剂同位素效应在特定酰基木瓜蛋白酶脱酰基反应中的作用。木瓜蛋白酶催化特定酯底物水解的质子库存研究：对可能的过渡态结构的分析。

Biochem J. 1981 Dec 1;199(3):681-92. doi: 10.1042/bj1990681.

Evidence that the lack of high catalytic activity of thiolsubtilisin towards specific substrates may be due to an inappropriately located proton-distribution system. Demonstration of highly nucleophilic character of the thiol group of thiolsubtilisin in the catalytically relevant ionization state of the active centre by use of a two-protonic-state reactivity probe.有证据表明，硫醇枯草杆菌蛋白酶对特定底物缺乏高催化活性可能是由于质子分布系统定位不当。通过使用双质子态反应性探针，证明了在活性中心的催化相关电离状态下硫醇枯草杆菌蛋白酶硫醇基团具有高度亲核性。

Biochem J. 1981 Mar 1;193(3):819-23. doi: 10.1042/bj1930819.

The interplay of electrostatic fields and binding interactions determining catalytic-site reactivity in actinidin. A possible origin of differences in the behaviour of actinidin and papain.静电场与结合相互作用的相互影响决定了猕猴桃蛋白酶催化位点的反应活性。猕猴桃蛋白酶和木瓜蛋白酶行为差异的一个可能来源。

Biochem J. 1989 Apr 15;259(2):443-52. doi: 10.1042/bj2590443.

Evidence for association-activation effects in reactions of papain from studies on its reactivity towards isomeric two-protonic-state reactivity probes.来自木瓜蛋白酶对异构双质子态反应性探针反应性研究的关联激活效应证据。

Biochem J. 1979 Nov 1;183(2):369-73. doi: 10.1042/bj1830369.

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