Thulborn K R, Sawyer W H
Biochim Biophys Acta. 1978 Aug 4;511(2):125-40. doi: 10.1016/0005-2736(78)90308-5.
The synthesis and properties of a set of four fluorescent probes (n-(9-anthroyloxy) fatty acids, n = 2, 6, 9, 12) sensitive to the fluidity gradient of the lipid bilayer are described. Fluorescent quenching experiments show that the probes locate at a graded series of depths in the bilayer. A fifth probe, methyl-9-anthroate, locates near the bilayer centre. As an example of their application, the probes are used to study the phase transitions of dipalmitoyl phosphatidyl-choline. Changes in the rotational relaxation times of the probes across the transitions are more pronounced at the centre of the bilayer than at the surface.
描述了一组对脂质双层流动性梯度敏感的四种荧光探针（n-(9-蒽氧基)脂肪酸，n = 2、6、9、12）的合成及性质。荧光猝灭实验表明，这些探针位于双层中一系列不同深度处。第五种探针，9-蒽甲酸甲酯，位于双层中心附近。作为其应用的一个例子,这些探针被用于研究二棕榈酰磷脂酰胆碱的相变。在相变过程中，探针旋转弛豫时间的变化在双层中心比在表面更为明显。
