Thulborn K R, Tilley L M, Sawyer W H, Treloar F E
Biochim Biophys Acta. 1979 Dec 4;558(2):166-78. doi: 10.1016/0005-2736(79)90057-9.
A set of n-(9-anthroyloxy) fatty acid probes (n = 2, 6, 9, 12) have been used to examine gradients in fluorescence polarization, lifetime (tau F), relative quantum yield (phi rel) and positions of emission maxima (lambda max) through bilayers composed of synthetic phospholipids. The fluorophores of these probes report the environment at a graded series of depths from the surface to the centre of the bilayer structure. 1. Polarizations decrease as the fluorophore is moved deeper into the bilayer indicating greater rotational motion of the fluorophore in the hydrocarbon core of the bilayer. 2. The different responses of the probe diphenylhexatriene and the anthroyloxy fatty acids to the action of cholesterol on lipid bilayers are discussed in terms of the orientation of these probes in the bilayer and the types of anisotropic rotational motions which result in depolarization of fluorescence. 3. Stearic acid derivatives which have the fluorophore in the 6-, 9- and 12-positions along the acyl chain have a similar response to solvent polarity as measured by values of lambda max and phi rel in a variety of organic solvents. 4. The position of the emission maximum has little dependence on solvent viscosity, but viscosity does change the degree of vibrational structure seen in the emission spectrum. The vibrational structure itself may be used as an indication of the 'mciroviscosity' gradient in the transverse plane of the bilayer. 5. Values of lambda max, tau F and phi rel indicate that a gradient of polarity exists from the surface to the centre of the bilayer. For dipalmitoyl phosphatidylcholine in the crystalline phase, cholesterol acts to make this polarity gradient shallower.
一组n-(9-蒽氧基)脂肪酸探针（n = 2、6、9、12）已被用于检测通过由合成磷脂组成的双层膜时，荧光偏振、寿命（τF）、相对量子产率（φrel）以及发射最大值位置（λmax）的梯度变化。这些探针的荧光团报告了从双层膜结构表面到中心一系列不同深度处的环境。1. 随着荧光团向双层膜内部移动，偏振度降低，这表明荧光团在双层膜烃核中的旋转运动增强。2. 根据这些探针在双层膜中的取向以及导致荧光去极化的各向异性旋转运动类型，讨论了探针二苯基己三烯和蒽氧基脂肪酸对胆固醇作用于脂质双层膜的不同响应。3. 荧光团位于酰基链6、9和12位的硬脂酸衍生物，在多种有机溶剂中，通过λmax和φrel值测量，对溶剂极性具有相似的响应。4. 发射最大值的位置对溶剂粘度的依赖性很小，但粘度确实会改变发射光谱中可见的振动结构程度。振动结构本身可作为双层膜横向平面中“微粘度”梯度的指示。5. λmax、τF和φrel值表明从双层膜表面到中心存在极性梯度。对于结晶相中的二棕榈酰磷脂酰胆碱，胆固醇会使这种极性梯度变浅。
