van den Hoogen Y T, van Beuzekom A A, de Vroom E, van der Marel G A, van Boom J H, Altona C
Gorlaeus Laboratories, Leiden University, The Netherlands.
Nucleic Acids Res. 1988 Jun 10;16(11):5013-30. doi: 10.1093/nar/16.11.5013.
Model-building studies were carried out on the trimer AUA. Bulge-out structures which allow incorporation into a continuous RNA helix were generated and energy-minimized. All geometrical features obtained by previous NMR studies on purine-pyrimidine-purine sequences are accounted for in these models. One of the models was used to fit into a double helical fragment. Only minor changes were necessary to construct a central bulge-out in an otherwise intact duplex. NMR and model-building studies were performed on the duplex (CUGGUGCGG).(CCGCCCAG) which contains an unpaired uridine residue. NOE data, chemical-shift profiles and imino-proton resonances provided evidence that the extra U is bulged out of the duplex. The relatively small dispersion in 31P chemical shifts (approximately equal to 0.7 ppm) indicate the absence of t/g or g/t combinations for the phosphodiester angles zeta/alpha. An energy-minimized model of the duplex, which fits the present collection of data, is presented.
对三聚体AUA进行了模型构建研究。生成了允许并入连续RNA螺旋的凸起结构，并进行了能量最小化处理。先前关于嘌呤-嘧啶-嘌呤序列的核磁共振研究获得的所有几何特征都在这些模型中得到了体现。其中一个模型被用于拟合一个双螺旋片段。在其他方面完整的双链体中构建一个中央凸起只需进行微小的改变。对含有未配对尿苷残基的双链体（CUGGUGCGG）·（CCGCCCAG）进行了核磁共振和模型构建研究。核Overhauser效应（NOE）数据、化学位移图谱和亚氨基质子共振提供了额外的U从双链体中凸起的证据。31P化学位移的相对较小分散（约等于0.7 ppm）表明磷酸二酯角ζ/α不存在t/g或g/t组合。本文给出了一个符合当前数据集的双链体能量最小化模型。
