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核糖戊核苷四磷酸在水溶液中的构象分析。500兆赫的二维核磁共振研究。

Conformational analysis of a ribopentanucleoside tetraphosphate in aqueous solution. A two-dimensional NMR study at 500 MHz.

作者信息

Lankhorst P P, Wille G, van Boom J H, Altona C, Haasnoot C A

出版信息

Nucleic Acids Res. 1983 May 11;11(9):2839-56. doi: 10.1093/nar/11.9.2839.

DOI:10.1093/nar/11.9.2839
PMID:6856477
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC325927/
Abstract

The 30 ribose proton resonances of the pentaribonucleoside tetraphosphate m6(2)AUm6(2)AUm6(2)A have been assigned unequivocally by means of spin-echo-correlated spectroscopy, 2D J-resolved spectroscopy and Nuclear Overhauser difference spectroscopy, carried out at 500 MHz. A detailed comparison of the conformational properties of the title compound with its constituent fragments m6(2)AUm6(2)AU, m6(2)AUm6(2)A, m6(2)AU and the relevant monomers is given. Chemical shift data indicate the existence of a doubly "bulged out" conformer, in which the two interior U-fragments are not involved in regular nearest neighbour stacking interactions. The coupling constants of the ribose-ring are interpreted in terms of the N/S equilibrium, and population distributions along the backbone angles beta and gamma are presented. The combined data suggest a strong similarity between the 5'-terminal triplets in m6(2)AUm6(2)AUm6(2)A, m6(2)AUm6(2)AU and m6(2)AUm6(2)A2.

摘要

通过在500兆赫下进行的自旋回波相关光谱、二维J分辨光谱和核Overhauser差异光谱，已明确指定了五核糖核苷四磷酸m6(2)AUm6(2)AUm6(2)A的30个核糖质子共振。给出了标题化合物与其组成片段m6(2)AUm6(2)AU、m6(2)AUm6(2)A、m6(2)AU和相关单体的构象性质的详细比较。化学位移数据表明存在一种双重“凸起”构象异构体，其中两个内部U片段不参与规则的最近邻堆积相互作用。根据N/S平衡解释了核糖环的耦合常数，并给出了沿主链角β和γ的种群分布。综合数据表明m6(2)AUm6(2)AUm6(2)A、m6(2)AUm6(2)AU和m6(2)AUm6(2)A2中5'-末端三联体之间有很强的相似性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7566/325927/452dac2c1174/nar00354-0336-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7566/325927/452dac2c1174/nar00354-0336-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7566/325927/452dac2c1174/nar00354-0336-a.jpg

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