Gray M W, Spencer D F
Nucleic Acids Res. 1981 Jul 24;9(14):3523-9. doi: 10.1093/nar/9.14.3523.
Küntzel et al. (1981) (Nucleic Acids Res. 9, 1451-1461) recently concluded that the sequence of wheat mitochondrial 5S rRNA is significantly more related to prokaryotic than to eukaryotic 5S rRNA sequences, and displays an especially high affinity to that of the thermophilic Gram-negative bacterium, Thermus aquaticus. However, the sequence on which this conclusion was based, although attributed to us, differs in several places from the one determined by us. We show here that the correct sequence (Spencer, D.F., Bonen, L. and Gray, M.W. (1981) Biochemistry, in press) does not support the conclusions of Küntzel et al. about potential secondary structure in wheat mitochondrial 5S rRNA and its phylogenetic significance. We further show that when the wheat mitochondrial 5S rRNA sequence is matched against published alignments for E. coli, T. aquaticus, and wheat cytosol 5S rRNAs, the mitochondrial sequence shows no greater homology to the T. aquaticus sequence than to the E. coli sequence, and only slightly more homology to these two sequences than to wheat cytosol 5S rRNA. This analysis confirms our original view (Biochemistry, in press) that wheat mitochondrial 5S rRNA is neither obviously prokaryotic nor eukaryotic in nature, but shows characteristics of both classes of 5S rRNA, as well as some unique features.
昆策尔等人（1981年）（《核酸研究》9卷，1451 - 1461页）最近得出结论，小麦线粒体5S核糖体RNA的序列与原核生物5S核糖体RNA序列的相关性明显高于与真核生物5S核糖体RNA序列的相关性，并且对嗜热革兰氏阴性细菌嗜热水生栖热菌的5S核糖体RNA序列表现出特别高的亲和力。然而，得出这一结论所依据的序列，尽管被认为是我们测定的，但在几个地方与我们测定的序列不同。我们在此表明，正确的序列（斯宾塞，D.F.，博嫩，L.和格雷，M.W.（1981年）《生物化学》，即将发表）并不支持昆策尔等人关于小麦线粒体5S核糖体RNA潜在二级结构及其系统发育意义的结论。我们进一步表明，当将小麦线粒体5S核糖体RNA序列与已发表的大肠杆菌、嗜热水生栖热菌和小麦细胞质5S核糖体RNA序列比对时，线粒体序列与嗜热水生栖热菌序列的同源性并不高于与大肠杆菌序列的同源性，并且与这两个序列的同源性仅比与小麦细胞质5S核糖体RNA序列略高。这一分析证实了我们原来的观点（《生物化学》，即将发表），即小麦线粒体5S核糖体RNA在本质上既不明显属于原核生物也不明显属于真核生物，而是兼具这两类5S核糖体RNA的特征，以及一些独特的特性。
