de Klerk G, Rosengarten P C, Vet R J, Goudsmit R
Blood. 1981 Dec;58(6):1164-70.
Erythropoietin (ESF) titers were determined in sera from patients with different types of anemia using the fetal mouse liver cell bioassay. An inverse relationship was found between hemoglobin concentration and ESF titer. However, ESF titers differed markedly between patients at comparable degrees of anemia. Several groups of patients were distinguished on the basis of the activity of their erythroid bone marrow. In each of these groups, a significant negative correlation was found between the hemoglobin concentration and the logarithm of the ESF titer. ESF titers in patients with pure red cell aplasia were fourfold higher than those in patients with iron-deficiency anemia and tenfold higher than those in patients with megaloblastic anemia and homozygous sickle cell anemia at comparable hemoglobin concentrations. Following the initiation of specific therapy in patients with pernicious anemia and patients wit iron-deficiency anemia, serum ESF titers were found to decrease prior to any substantial rise in hemoglobin concentrations. In the patients with pernicious anemia, the lowest ESF levels were found 1 day after administration of vitamin B12, whereas in the patients with iron-deficiency anemia, the lowest ESF levels were reached in the second week of oral iron therapy. ON the basis of these data it was concluded that serum ESF titers in anemic patients are not only inversely related to the hemoglobin concentration but also to the activity of the erythroid bone marrow.
使用胎鼠肝细胞生物测定法测定了不同类型贫血患者血清中的促红细胞生成素（ESF）滴度。发现血红蛋白浓度与ESF滴度呈负相关。然而，在贫血程度相当的患者中，ESF滴度存在显著差异。根据红系骨髓活性区分出几组患者。在这些组中的每一组中，血红蛋白浓度与ESF滴度的对数之间都存在显著的负相关。在血红蛋白浓度相当的情况下，纯红细胞再生障碍性贫血患者的ESF滴度比缺铁性贫血患者高四倍，比巨幼细胞贫血和纯合子镰状细胞贫血患者高十倍。在恶性贫血患者和缺铁性贫血患者开始进行特异性治疗后，发现血清ESF滴度在血红蛋白浓度大幅升高之前就降低了。在恶性贫血患者中，在给予维生素B12后1天发现ESF水平最低，而在缺铁性贫血患者中，口服铁剂治疗的第二周达到最低ESF水平。根据这些数据得出结论，贫血患者的血清ESF滴度不仅与血红蛋白浓度呈负相关，而且与红系骨髓活性有关。
