酶联免疫吸附测定法与免疫沉淀法用于脑膜炎奈瑟菌血清分型的比较及其在证明血清型多态性中的应用
Enzyme-linked immunosorbent assay compared with immunoprecipitation for serotyping Neisseria meningitidis and its use in demonstrating serotype polymorphism.
作者信息
Mackie E B, White L A, Brown K N, Bryan L E
出版信息
J Clin Microbiol. 1981 Mar;13(3):449-55. doi: 10.1128/jcm.13.3.449-455.1981.
Abstract
Rabbit antisera generated against 11 prototype strains of group B Neisseria meningitidis were tritiated by using the enzyme-linked immunosorbent assay and the Ouchterlony immunoprecipitation technique. The relative insensitivity of the immunoprecipitation technique in detecting antibody compared with the enzyme-linked immunosorbent assay was demonstrated. Cross-reactions within the group and among the 11 serotypes were characterized with respect to established serological techniques. Serotype polymorphism among the strains of N. meningitidis was demonstrated with the enzyme-linked immunosorbent assay.
摘要
利用酶联免疫吸附测定法和双向免疫扩散免疫沉淀技术，对针对11种B群脑膜炎奈瑟菌原型菌株产生的兔抗血清进行了氚标记。结果表明，与酶联免疫吸附测定法相比，免疫沉淀技术在检测抗体方面相对不敏感。根据既定的血清学技术，对该组内以及11种血清型之间的交叉反应进行了表征。利用酶联免疫吸附测定法证明了脑膜炎奈瑟菌菌株之间的血清型多态性。
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