Sugasawara R J, Prato C, Sippel J E
Infect Immun. 1983 Dec;42(3):863-8. doi: 10.1128/iai.42.3.863-868.1983.
A cell line producing monoclonal antibodies directed against a lipopolysaccharide component of Neisseria meningitidis group A has been established. These antibodies reacted with only one of three lipopolysaccharide serotyping strains of group A meningococci by coagglutination, enzyme-linked immunosorbent assay, and Western blotting techniques. A Western blot analysis showed that a NaOH digest of lipopolysaccharide was detectable by the serotype-specific antibody. The monoclonal antibodies cross-reacted with a group B meningococcal strain in an enzyme-linked immunosorbent assay. The immunoblotting analysis also showed that these antibodies reacted with the lipopolysaccharides of a group B meningococcus as well as Haemophilus influenzae type B, but not with the lipopolysaccharides of several strains of Salmonella typhi, Escherichia coli, Streptococcus pneumoniae, and Neisseria gonorrhoeae.
已建立了一种细胞系，该细胞系可产生针对A群脑膜炎奈瑟菌脂多糖成分的单克隆抗体。通过协同凝集、酶联免疫吸附测定和蛋白质印迹技术，这些抗体仅与A群脑膜炎球菌的三种脂多糖血清分型菌株之一发生反应。蛋白质印迹分析表明，血清型特异性抗体可检测到脂多糖的NaOH消化产物。在酶联免疫吸附测定中，这些单克隆抗体与B群脑膜炎球菌菌株发生交叉反应。免疫印迹分析还表明，这些抗体与B群脑膜炎球菌以及B型流感嗜血杆菌的脂多糖发生反应，但不与伤寒沙门氏菌、大肠杆菌、肺炎链球菌和淋病奈瑟菌的几种菌株的脂多糖发生反应。
