Siddiqi S H, Libonati J P, Middlebrook G
J Clin Microbiol. 1981 May;13(5):908-12. doi: 10.1128/jcm.13.5.908-912.1981.
A total of 106 isolates of Mycobacterium tuberculosis were tested for drug susceptibility by the conventional 7H11 plate method and by a new rapid radiometric method using special 7H12 liquid medium with 14C-labeled substrate. Results obtained by the two methods were compared for rapidity, sensitivity, and specificity of the new test method. There was 98% overall agreement between the results obtained by the two methods. Of a total of 424 drug tests, only 8 drug results did not agree, mostly in the case of streptomycin. This new procedure was found to be rapid, with 87% of the tests results reportable within 4 days and 98% reportable within 5 days as compared to the usual 3 weeks required with the conventional indirect susceptibility test method. The results of this preliminary study indicate that the rapid radiometric method seems to have the potential for routine laboratory use and merits further investigations.
采用传统的7H11平板法和一种使用含14C标记底物的特殊7H12液体培养基的新型快速放射测量法，对总共106株结核分枝杆菌进行了药敏试验。比较了两种方法在新检测方法的快速性、敏感性和特异性方面所获得的结果。两种方法所获结果的总体一致性为98%。在总共424次药敏试验中，只有8次药敏结果不一致，主要是链霉素的情况。发现这种新方法速度很快，与传统间接药敏试验方法通常需要的3周时间相比，87%的试验结果可在4天内报告，98%可在5天内报告。这项初步研究的结果表明，快速放射测量法似乎有在常规实验室使用的潜力，值得进一步研究。
