Veselý D, Veselá D
Vet Med (Praha). 1981 Feb;26(2):109-15.
Feeding the maize silage infected with the mold Penicillium roqueforti resulted in 112 dairy cows in the loss of appetite, stoppage of rumen activity and gut inflammation. The abortion of first-calvers in the seventh and eighth month was observed. The mold P. roqueforti produced on the sterile maize silage, at the temperature of 20 degrees C, up to 160 mg of PR-toxin per kg after 22 days of cultivation. The maximum production in the liquid medium was 900 mg of PR-toxin per litre, at the temperature of 13 degrees C after 50 days of cultivation. The isolated PR-toxin was identified spectrophotometrically and by the thin-layer chromatography after detection by 3-methyl-2-benzothiazoline-hydrochloride. The dose of 0.01 microgram PR-toxin was extremely toxic for 40-hr old chicken embryos.U
给感染了罗克福特青霉的玉米青贮饲料喂食，导致112头奶牛出现食欲不振、瘤胃活动停止和肠道炎症。观察到初产母牛在第七和第八个月出现流产。罗克福特青霉在无菌玉米青贮饲料上，于20摄氏度培养22天后，每千克可产生高达160毫克的PR毒素。在液体培养基中，于13摄氏度培养50天后，最大产量为每升900毫克PR毒素。通过3 - 甲基 - 2 - 苯并噻唑啉 - 盐酸盐检测后，采用分光光度法和薄层色谱法对分离出的PR毒素进行鉴定。0.01微克PR毒素的剂量对40小时龄的鸡胚具有极高毒性。
