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纤维二糖利用相关基因的分子克隆及其在大肠杆菌中的表达。

Molecular cloning of genes for cellobiose utilization and their expression in Escherichia coli.

作者信息

Armentrout R W, Brown R D

出版信息

Appl Environ Microbiol. 1981 Jun;41(6):1355-62. doi: 10.1128/aem.41.6.1355-1362.1981.

DOI:10.1128/aem.41.6.1355-1362.1981
PMID:6787983
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC243923/
Abstract

The genes for cellobiose utilization in Escherichia adecarboxylata were cloned by using recombinant deoxyribonucleic acid techniques and transferred to Escherichia coli. Preliminary analysis of the beta-glucosidase activity expressed in these host cells indicated that the enzyme is membrane bound and required magnesium ions, phosphate ions, and heat-stable, non-dialyzable factors from the bacterial cytoplasm.

摘要

利用重组脱氧核糖核酸技术克隆了非脱羧埃希氏菌中利用纤维二糖的基因，并将其转移到大肠杆菌中。对这些宿主细胞中表达的β-葡萄糖苷酶活性的初步分析表明，该酶与膜结合，并且需要镁离子、磷酸根离子以及来自细菌细胞质的热稳定、不可透析的因子。

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本文引用的文献

1
Partial sequence analysis of Xenopus alpha- and beta-globin mRNA as determined from recombinant DNA plasmids.从重组DNA质粒中测定非洲爪蟾α-和β-珠蛋白mRNA的部分序列分析。
Dev Biol. 1980 Jul;78(1):161-72. doi: 10.1016/0012-1606(80)90326-7.
2
A complementation analysis of the restriction and modification of DNA in Escherichia coli.大肠杆菌中DNA限制与修饰的互补分析。
J Mol Biol. 1969 May 14;41(3):459-72. doi: 10.1016/0022-2836(69)90288-5.
3
General method for the isolation of plasmid deoxyribonucleic acid.质粒脱氧核糖核酸的分离通用方法。
J Bacteriol. 1973 Nov;116(2):1064-6. doi: 10.1128/jb.116.2.1064-1066.1973.
4
The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts.通过渗透休克以及在原生质体形成过程中从大肠杆菌释放酶。
J Biol Chem. 1965 Sep;240(9):3685-92.
5
Active transport of maltose in Escherichia coli K12. Involvement of a "periplasmic" maltose binding protein.大肠杆菌K12中麦芽糖的主动运输。一种“周质”麦芽糖结合蛋白的作用。
Eur J Biochem. 1974 Aug 15;47(1):139-49. doi: 10.1111/j.1432-1033.1974.tb03677.x.