纤维二糖利用相关基因的分子克隆及其在大肠杆菌中的表达。
Molecular cloning of genes for cellobiose utilization and their expression in Escherichia coli.
作者信息
Armentrout R W, Brown R D
出版信息
Appl Environ Microbiol. 1981 Jun;41(6):1355-62. doi: 10.1128/aem.41.6.1355-1362.1981.
Abstract
The genes for cellobiose utilization in Escherichia adecarboxylata were cloned by using recombinant deoxyribonucleic acid techniques and transferred to Escherichia coli. Preliminary analysis of the beta-glucosidase activity expressed in these host cells indicated that the enzyme is membrane bound and required magnesium ions, phosphate ions, and heat-stable, non-dialyzable factors from the bacterial cytoplasm.
摘要
利用重组脱氧核糖核酸技术克隆了非脱羧埃希氏菌中利用纤维二糖的基因，并将其转移到大肠杆菌中。对这些宿主细胞中表达的β-葡萄糖苷酶活性的初步分析表明，该酶与膜结合，并且需要镁离子、磷酸根离子以及来自细菌细胞质的热稳定、不可透析的因子。
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