膜醭假丝酵母β-葡萄糖苷酶基因的核苷酸序列
Nucleotide sequence of Candida pelliculosa beta-glucosidase gene.
作者信息
Kohchi C, Toh-e A
出版信息
Nucleic Acids Res. 1985 Sep 11;13(17):6273-82. doi: 10.1093/nar/13.17.6273.
Abstract
The nucleotide sequence of the DNA fragment containing the beta-glucosidase gene of Candida pelliculosa was determined. Analysis of the sequence revealed three open reading frames which could encode 65,825, and 412 amino acid residues. The presence of the second frame was found to be sufficient for the expression of the beta-glucosidase gene in a heterologous host Saccharomyces cerevisiae. Putative protein encoded by this gene had hydrophobic amino acids, resembling a signal peptide, at its N-terminal region and 19 potential glycosylation sites. Codon usage of Candida genes had the similar pattern shown in S.cerevisiae. Codon bias of the beta-glucosidase gene of Candida was relatively low, compared with that of the highly expressed genes of S. cerevisiae.
摘要
测定了包含膜醭假丝酵母β-葡萄糖苷酶基因的DNA片段的核苷酸序列。序列分析揭示了三个开放阅读框，它们可编码65、825和412个氨基酸残基。发现第二个阅读框的存在足以使β-葡萄糖苷酶基因在异源宿主酿酒酵母中表达。该基因编码的推定蛋白质在其N端区域具有疏水性氨基酸，类似于信号肽，并有19个潜在的糖基化位点。膜醭假丝酵母基因的密码子使用模式与酿酒酵母中所示的模式相似。与酿酒酵母高表达基因相比，膜醭假丝酵母β-葡萄糖苷酶基因的密码子偏好性相对较低。
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