Tomita T, Blumenstock E, Kanegasaki S
Infect Immun. 1981 Jun;32(3):1242-8. doi: 10.1128/iai.32.3.1242-1248.1981.
In the presence of luminol, resident as well as thioglycolate-induced and immunized macrophages emitted chemiluminescence more efficiently when the cells were exposed to living Salmonella typhimurium than when they were exposed to the same bacterium killed by ultraviolet light or heat. This phenomenon was observed whether or not the bacterium was opsonized. The different response to living and killed bacteria was also found with Escherichia coli, Pseudomonas aeruginosa, Proteus morganii, and Enterobacter aerogenes, but not with Shigella sonnei, Klebsiella pneumoniae, and Propionibacterium acnes. The results suggest that macrophages respond better to living, motile bacteria than to nonmotile or killed bacteria. The experimental results obtained with motility mutants of S. typhimurium, E. coli, and P. aeruginosa confirm that macrophages exposed to the motile bacteria emit chemiluminescence more efficiently and ingest the motile bacteria at a much faster rate than the nonmotile bacteria.
在鲁米诺存在的情况下，当细胞暴露于活的鼠伤寒沙门氏菌时，驻留巨噬细胞以及巯基乙酸盐诱导的和免疫的巨噬细胞发出化学发光的效率比暴露于经紫外线或加热杀死的相同细菌时更高。无论细菌是否被调理，都观察到了这种现象。在大肠杆菌、铜绿假单胞菌、摩根氏变形杆菌和气杆菌中也发现了对活细菌和死细菌的不同反应，但宋内志贺氏菌、肺炎克雷伯菌和痤疮丙酸杆菌没有这种反应。结果表明，巨噬细胞对活的、有运动能力的细菌的反应比对无运动能力或已杀死的细菌更好。用鼠伤寒沙门氏菌、大肠杆菌和铜绿假单胞菌的运动突变体获得的实验结果证实，暴露于有运动能力细菌的巨噬细胞发出化学发光的效率更高，摄取有运动能力细菌的速度比无运动能力细菌快得多。
