在T细胞中寻找携带免疫球蛋白VH核苷酸序列的信使RNA分子。
A search for messenger RNA molecules bearing immunoglobulin VH nucleotide sequences in T cells.
作者信息
Kemp D J, Adams J M, Mottram P L, Thomas W R, Walker I D, Miller J F
出版信息
J Exp Med. 1982 Dec 1;156(6):1848-53. doi: 10.1084/jem.156.6.1848.
Abstract
Expression of VH-coded mRNA molecules in T cells, antigen-specific T cell lines, or T cell hybridomas was not detected using four different VH DNA probes under conditions that permitted cross-hybridization between distantly related VH genes. In contrast, VH gene expression was readily detected in two B cell lymphomas and in splenic B cells. Less than one molecule per cell of RNA, exactly complementary to the DNA probes used, would have been detected in these T cell populations. The results thus seriously question the proposition that T cells use the B cell VH repertoire to code for antigen receptors.
摘要
在允许远缘相关VH基因之间交叉杂交的条件下，使用四种不同的VH DNA探针未检测到T细胞、抗原特异性T细胞系或T细胞杂交瘤中VH编码的mRNA分子的表达。相比之下，在两种B细胞淋巴瘤和脾B细胞中很容易检测到VH基因表达。在这些T细胞群体中，每个细胞中与所用DNA探针完全互补的RNA分子不到一个。因此，这些结果严重质疑了T细胞利用B细胞VH基因库编码抗原受体这一观点。
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