Gottschalk J, Kunz J, Barthelmes H, Biester I, Kreher C
Acta Histochem Suppl. 1980;22:349-56.
After the intraperitoneal application of 3H-proline a higher grain density over the myocardium and the aorta in comparison with the cartilage, the glomeruli and the cutaneous connective tissue of rats was found. The maximal incorporation was established in the aorta at 6 h, in the skeletal muscle at 18 h after the injection of radioactive proline. Immunhistochemical findings reported in the literature and first results of our own indicate a special distribution pattern of the various collagen types in the organs under study. Seen in this connection the autoradiographic results may imply that type I and III collagen has a higher metabolic intensity than type II and IV collagen. Animals with renal hypertension of 3 weeks duration show an increase in the ratio of the extranuclear to the intranuclear grain density in the myocardium and the aorta after 3H-proline application.
腹腔注射3H-脯氨酸后，发现与大鼠的软骨、肾小球和皮肤结缔组织相比，心肌和主动脉上的银粒密度更高。注射放射性脯氨酸后6小时，主动脉中出现最大掺入量，18小时后在骨骼肌中出现最大掺入量。文献报道的免疫组织化学结果以及我们自己的初步结果表明，在所研究的器官中，各种胶原类型具有特殊的分布模式。由此看来，放射自显影结果可能意味着I型和III型胶原比II型和IV型胶原具有更高的代谢强度。持续3周肾性高血压的动物在应用3H-脯氨酸后，心肌和主动脉中核外与核内银粒密度之比增加。
