Saavedra J M, Setler P E, Kebabian J W
Brain Res. 1978 Aug 4;151(2):339-52. doi: 10.1016/0006-8993(78)90889-2.
The biochemical consequences of a unilateral 6-hydroxydopamine injection into the substantia nigra of the rat brain were investigated. Projections of dopaminergic neurons from the A8-A9-A10 regions to a number of forebrain areas were confirmed. No innervation to the hypothalamus, including the median eminence, or to the brain stem, could be found with the present techniques. No destruction of serotonergic or GABAergic fibers could be demonstrated in the lesioned substantia nigra. Increases in glutamic acid decarboxylase activity were found restricted to the caudate and zona compacta of the substantia nigra ipsilateral to the lesion, indicating the possibility of a physiological interaction between GABAergic and dopaminergic systems. The neuroanatomical localization of the nigral dopamine-sensitive adenylate cyclase was also studied. No change in enzyme activity was found after destruction of a great proportion of the dopaminergic cells, suggesting that this enzyme has an extradopaminergic localization in the substantia nigra.
研究了向大鼠脑黑质单侧注射6-羟基多巴胺的生化后果。证实了多巴胺能神经元从A8-A9-A10区域向多个前脑区域的投射。用现有技术未发现向包括正中隆起在内的下丘脑或脑干的神经支配。在受损黑质中未发现5-羟色胺能或γ-氨基丁酸能纤维的破坏。发现谷氨酸脱羧酶活性的增加仅限于损伤同侧的尾状核和黑质致密部，表明γ-氨基丁酸能和多巴胺能系统之间存在生理相互作用的可能性。还研究了黑质多巴胺敏感腺苷酸环化酶的神经解剖定位。在大部分多巴胺能细胞被破坏后，未发现酶活性有变化，这表明该酶在黑质中有一个多巴胺能外的定位。
