Gillies R J, Rosenberg M P, Deamer D W
J Cell Physiol. 1981 Aug;108(2):115-22. doi: 10.1002/jcp.1041080202.
"Fertilization acid" is released from sea urchin eggs upon fertilization and decreases the pH of the surrounding seawater. In bicarbonate-free artificial seawater flushed with nitrogen gas, the pH shift still occurs but returns to the original value in a few minutes, suggesting that the released acid volatile. A likely candidate for a volatile acid is carbon dioxide released from the eggs. Therefore, the total CO2 content of seawater was measured pre- and post-fertilization and was found to be correlated stoichiometrically with released proton equivalents, leading to the conclusion that fertilization acid is largely carbon dioxide. Manometric analysis of cell extracts and ashed eggs suggest that the carbon dioxide may be stored in the unfertilized egg as an inorganic carbonate.
“受精酸”在海胆卵受精时从卵中释放出来，会降低周围海水的pH值。在用氮气冲洗过的无碳酸氢盐人工海水中，pH值仍会发生变化，但几分钟后会恢复到初始值，这表明释放出的酸是挥发性的。挥发性酸的一个可能候选物是卵释放出的二氧化碳。因此，测量了受精前后海水的总二氧化碳含量，发现其与释放的质子当量呈化学计量关系，从而得出受精酸主要是二氧化碳的结论。对细胞提取物和灰化卵的测压分析表明，二氧化碳可能以无机碳酸盐的形式储存在未受精的卵中。
