Area de Microbiología, EPSO, Universidad Miguel Hernández, 03312, Orihuela, Alicante, Spain.
Genetic PCR Solutions TM, CEEI, Elche, Alicante, Spain.
Curr Microbiol. 2016 Jun;72(6):795-803. doi: 10.1007/s00284-016-0997-9. Epub 2016 Feb 12.
During previous studies to evaluate the phylogenetic diversity of Aeromonas from untreated waters and vegetables intended for human consumption, a group of isolates formed a unique gyrB phylogenetic cluster, separated from those of all other species described so far. A subsequent extensive phenotypic characterization, DNA-DNA hybridization, 16S rRNA gene sequencing, multi-locus phylogenetic analysis of the concatenated sequence of seven housekeeping genes (gyrB, rpoD, recA, dnaJ, gyrA, dnaX, and atpD; 4705 bp), and ERIC-PCR, were performed in an attempt to ascertain the taxonomy position of these isolates. This polyphasic approach confirmed that they belonged to a novel species of the genus Aeromonas, for which the name Aeromonas lusitana sp. nov. is proposed, with strain A.11/6(T) (=DSMZ 24095(T), =CECT 7828(T)) as the type strain.
在之前的研究中,评估未经处理的水和供人类食用的蔬菜中的气单胞菌的系统发育多样性时,一组分离株形成了一个独特的gyrB 系统发育群,与迄今为止描述的所有其他种分离株都不同。随后,对这些分离株进行了广泛的表型特征描述、DNA-DNA 杂交、16S rRNA 基因测序、7 个看家基因(gyrB、rpoD、recA、dnaJ、gyrA、dnaX 和 atpD;4705bp)的串联序列多基因系统发育分析和 ERIC-PCR,以确定这些分离株的分类地位。这种多相方法证实它们属于气单胞菌属的一个新种,因此提议将其命名为 Aeromonas lusitana sp. nov.,其模式菌株为 A.11/6(T)(=DSMZ 24095(T), =CECT 7828(T))。