Budínská O, Kubín V, Franĕk J, Julák J, Panos J
Folia Microbiol (Praha). 1981;26(3):212-6. doi: 10.1007/BF02927426.
A lipopolysaccharide was isolated by extraction of Aspergillus flavus conidia with 45% phenol at 68-70 degrees C. Quantitative analysis revealed 7% nucleic acids, 5.5% proteins, 46% polysaccharides and 49% liquids, of which 12% were covalently bound. Glucose, mannose, galactose and fucose were detected as monosaccharide components of the polysaccharide moiety by gas chromatography; palmitic acid, stearic acid, oleic acid, linoleic acid and myristic acid were mainly present in the lipidic fraction. This material differs from the bacterial lipopolysaccharides, both in composition of the polysaccharide moiety and representation of fatty acids in the lipidic fraction.
通过在68 - 70摄氏度下用45%的苯酚提取黄曲霉分生孢子,分离出一种脂多糖。定量分析显示,该脂多糖含有7%的核酸、5.5%的蛋白质、46%的多糖和49%的液体,其中12%为共价结合。通过气相色谱法检测到葡萄糖、甘露糖、半乳糖和岩藻糖是多糖部分的单糖成分;棕榈酸、硬脂酸、油酸、亚油酸和肉豆蔻酸主要存在于脂质部分。这种物质在多糖部分的组成和脂质部分脂肪酸的表现形式上均与细菌脂多糖不同。