利用基因融合技术研究半胱氨酸调节子的调控基因cysB的表达。
Use of gene fusions to study expression of cysB, the regulatory gene of the cysteine regulon.
作者信息
Jagura-Burdzy G, Hulanicka D
出版信息
J Bacteriol. 1981 Sep;147(3):744-51. doi: 10.1128/jb.147.3.744-751.1981.
Abstract
Strains of escherichia coli were constructed in which the lacZ gene is fused to cysB, the positive regulator gene of the cysteine regulon. The fusion strains were used to study the regulation of the cysB gene by assaying the fused lacZ gene product. The introduction of a cysB allele, either on a plasmid or on an episome to the fusion strains, resulted in the decrease of beta-galactosidase activity. This implies that the cysB gene expression is autoregulated by its own product. The direction of cysB gene transcription was determined to be clockwise.
摘要
构建了大肠杆菌菌株，其中lacZ基因与半胱氨酸调节子的正调控基因cysB融合。通过检测融合的lacZ基因产物，利用这些融合菌株来研究cysB基因的调控。将cysB等位基因（无论是在质粒上还是在附加体上）导入融合菌株，导致β-半乳糖苷酶活性降低。这意味着cysB基因的表达受其自身产物的自动调节。确定cysB基因转录方向为顺时针。
相似文献
1
Use of gene fusions to study expression of cysB, the regulatory gene of the cysteine regulon.利用基因融合技术研究半胱氨酸调节子的调控基因cysB的表达。
J Bacteriol. 1981 Sep;147(3):744-51. doi: 10.1128/jb.147.3.744-751.1981.
2
Effect of DNA gyrase inhibitors and urea on the expression of cysB, the regulatory gene of the cysteine regulon.DNA 回旋酶抑制剂和尿素对半胱氨酸调节子的调节基因cysB表达的影响。
J Gen Microbiol. 1986 Sep;132(9):2571-6. doi: 10.1099/00221287-132-9-2571.
3
Regulation of the cysB gene expression in Escherichia coli.大肠杆菌中cysB基因表达的调控
Acta Biochim Pol. 1986;33(2):133-7.
4
Effect of mutations in dnaK and dnaJ genes on cysteine operon expression in Escherichia coli.dnaK和dnaJ基因突变对大肠杆菌中半胱氨酸操纵子表达的影响。
Folia Microbiol (Praha). 2002;47(4):371-4. doi: 10.1007/BF02818692.
5
Identification of the CysB-regulated gene, hslJ, related to the Escherichia coli novobiocin resistance phenotype.与大肠杆菌新生霉素抗性表型相关的CysB调控基因hslJ的鉴定。
FEMS Microbiol Lett. 2003 Jul 29;224(2):239-46. doi: 10.1016/S0378-1097(03)00441-5.
6
Analysis of the Escherichia coli K-12 cysB gene and its product using the method of gene fusion.
Biochem Biophys Res Commun. 1990 Mar 30;167(3):962-9. doi: 10.1016/0006-291x(90)90617-v.
7
Regulation of expression of the uxu operon and of the uxuR regulatory gene in Escherichia coli K12.大肠杆菌K12中uxu操纵子及uxuR调控基因表达的调控
J Gen Microbiol. 1983 Nov;129(11):3345-53. doi: 10.1099/00221287-129-11-3345.
8
The LysR-type transcriptional regulator CysB controls the repression of hslJ transcription in Escherichia coli.LysR 型转录调节因子 CysB 控制大肠杆菌中 hslJ 转录的抑制。
Microbiology (Reading). 2003 Dec;149(Pt 12):3449-3459. doi: 10.1099/mic.0.26609-0.
9
opp-lac Operon fusions and transcriptional regulation of the Escherichia coli trp-linked oligopeptide permease.大肠杆菌色氨酸连接寡肽通透酶的opp-lac操纵子融合与转录调控
J Bacteriol. 1986 Feb;165(2):434-42. doi: 10.1128/jb.165.2.434-442.1986.
10
Use of in vitro gene fusions to study the uxuR regulatory gene in Escherichia coli K-12: direction of transcription and regulation of its expression.利用体外基因融合技术研究大肠杆菌K-12中的uxuR调控基因：转录方向及其表达调控
J Bacteriol. 1982 Jun;150(3):1040-7. doi: 10.1128/jb.150.3.1040-1047.1982.
引用本文的文献
1
Uncovering Key Metabolic Determinants of the Drug Interactions Between Trimethoprim and Erythromycin in .揭示甲氧苄啶与红霉素在……中药物相互作用的关键代谢决定因素。
Front Microbiol. 2021 Oct 20;12:760017. doi: 10.3389/fmicb.2021.760017. eCollection 2021.
2
Unique Properties of the Alpha-Helical DNA-Binding Protein KfrA Encoded by the IncU Incompatibility Group Plasmid RA3 and Its Host-Dependent Role in Plasmid Maintenance.由 IncU 不相容群质粒 RA3 编码的α-螺旋 DNA 结合蛋白 KfrA 的独特性质及其在质粒维持中的宿主依赖性作用。
Appl Environ Microbiol. 2021 Jan 4;87(2). doi: 10.1128/AEM.01771-20.
3
Transcriptional Organization of the Stability Module of Broad-Host-Range Plasmid RA3, from the IncU Group.IncU 组广谱质粒 RA3 的稳定性模块的转录组织。
Appl Environ Microbiol. 2020 Aug 3;86(16). doi: 10.1128/AEM.00847-20.
4
Oleanolic and ursolic acids influence affect the expression of the cysteine regulon and the stress response in Escherichia coli.齐墩果酸和熊果酸影响影响大肠埃希氏菌半胱氨酸调控基因和应激反应的表达。
Curr Microbiol. 2011 Apr;62(4):1331-6. doi: 10.1007/s00284-010-9866-0. Epub 2011 Jan 9.
5
Effect of mutations in dnaK and dnaJ genes on cysteine operon expression in Escherichia coli.dnaK和dnaJ基因突变对大肠杆菌中半胱氨酸操纵子表达的影响。
Folia Microbiol (Praha). 2002;47(4):371-4. doi: 10.1007/BF02818692.
6
Identification of Bacillus subtilis CysL, a regulator of the cysJI operon, which encodes sulfite reductase.枯草芽孢杆菌CysL的鉴定，CysL是cysJI操纵子的一个调控因子，该操纵子编码亚硫酸盐还原酶。
J Bacteriol. 2002 Sep;184(17):4681-9. doi: 10.1128/JB.184.17.4681-4689.2002.
7
Deletion analysis of the Escherichia coli taurine and alkanesulfonate transport systems.大肠杆菌牛磺酸和链烷磺酸盐转运系统的缺失分析
J Bacteriol. 2000 May;182(10):2687-95. doi: 10.1128/JB.182.10.2687-2695.2000.
8
Linkage map of Escherichia coli K-12, edition 10: the traditional map.大肠杆菌K-12连锁图谱，第10版：传统图谱。
Microbiol Mol Biol Rev. 1998 Sep;62(3):814-984. doi: 10.1128/MMBR.62.3.814-984.1998.
9
Involvement of CysB and Cbl regulatory proteins in expression of the tauABCD operon and other sulfate starvation-inducible genes in Escherichia coli.CysB和Cbl调节蛋白参与大肠杆菌中tauABCD操纵子及其他硫酸盐饥饿诱导基因的表达。
J Bacteriol. 1997 Dec;179(24):7671-8. doi: 10.1128/jb.179.24.7671-7678.1997.
10
Characterization of the CysB protein of Klebsiella aerogenes: direct evidence that N-acetylserine rather than O-acetylserine serves as the inducer of the cysteine regulon.产气克雷伯菌CysB蛋白的特性：直接证据表明N - 乙酰丝氨酸而非O - 乙酰丝氨酸作为半胱氨酸调节子的诱导物。
Biochem J. 1994 Apr 1;299 ( Pt 1)(Pt 1):129-36. doi: 10.1042/bj2990129.
本文引用的文献
1
METABOLIC REGULATION OF ADENOSINE TRIPHOSPHATE SULFURYLASE IN YEAST.酵母中三磷酸腺苷硫酸化酶的代谢调控
J Bacteriol. 1964 Nov;88(5):1341-8. doi: 10.1128/JB.88.5.1341-1348.1964.
2
Hybridization between Escherichia coli and Shigella.大肠杆菌与志贺氏菌之间的杂交。
J Bacteriol. 1957 Oct;74(4):461-76. doi: 10.1128/jb.74.4.461-476.1957.
3
Acetylornithinase of Escherichia coli: partial purification and some properties.大肠杆菌的乙酰鸟氨酸酶：部分纯化及某些性质
J Biol Chem. 1956 Jan;218(1):97-106.
4
5
The purification and characterization of O-acetylserine sulfhydrylase-A from Salmonella typhimurium.鼠伤寒沙门氏菌O-乙酰丝氨酸巯基酶-A的纯化与特性分析
J Biol Chem. 1969 May 10;244(9):2418-27.
6
Positive control of sulphate reduction in Escherichia coli. The nature of the pleiotropic cysteineless mutants of E. coli K12.大肠杆菌中硫酸盐还原的正调控。大肠杆菌K12多效性无半胱氨酸突变体的性质。
Biochem J. 1968 Dec;110(3):597-602. doi: 10.1042/bj1100597.
7
Inhibition of lacZ gene translation initiation in trp-lac fusion strains.色氨酸-乳糖操纵子融合菌株中lacZ基因翻译起始的抑制作用。
J Bacteriol. 1974 Mar;117(3):1231-9. doi: 10.1128/jb.117.3.1231-1239.1974.
8
Transduction by bacteriophage MU-1.
Virology. 1973 Sep;55(1):103-17. doi: 10.1016/s0042-6822(73)81012-8.
9
Prophage deletion mapping of bacteriophage Mu-1.噬菌体Mu-1的原噬菌体缺失图谱分析
Virology. 1973 Jul;54(1):93-101. doi: 10.1016/0042-6822(73)90118-9.
10
Role of molecular conformation in determining the electrophoretic properties of polynucleotides in agarose-acrylamide gels. II.分子构象在决定琼脂糖-丙烯酰胺凝胶中多核苷酸电泳性质方面的作用。II.
Biochemistry. 1972 Mar 28;11(7):1242-50. doi: 10.1021/bi00757a020.
Zotero 插件