Mason D Y, Stein H, Naiem M, Abdulaziz Z
J Cancer Res Clin Oncol. 1981;101(1):13-22. doi: 10.1007/BF00405059.
The increasing number of antigens detectable in human lymphoid tissue (particularly since the advent of monoclonal antibodies) makes it necessary to have techniques available for studying the relative distribution patterns of pairs of antigens in tissue sections. Double immunoenzymatic labelling (using peroxidase and alkaline phosphatase) offers a number of advantages over double immunofluorescence, including the fact that the two antigens can be visualised simultaneously (rather than sequentially) and that the labels are permanent. In studying paraffin-embedded human lymphoid tissue an important application of the double immunoenzymatic technique lies in distinguishing Ig-positive cells containing exogenous Ig (which stain for only a single light chain class). In addition double staining of paraffin sections for IgG and IgM has been used to show that "switch" cells containing both these classes of heavy chain are rare in reactive lymphoid tissue. The potential scope of the double immunoenzymatic technique has been extended by showing that the procedure is applicable to cryostat sections (in which antigenic reactivity is better preserved than in paraffin sections) and by adapting it for use with monoclonal antibodies (by preparing "monoclonal PAP" complexes).
在人类淋巴组织中可检测到的抗原数量不断增加（特别是自单克隆抗体问世以来），这使得有必要具备可用于研究组织切片中抗原对相对分布模式的技术。与双重免疫荧光相比，双重免疫酶标记法（使用过氧化物酶和碱性磷酸酶）具有诸多优势，包括可以同时（而非顺序地）观察两种抗原，并且标记是永久性的。在研究石蜡包埋的人类淋巴组织时，双重免疫酶技术的一个重要应用在于区分含有外源性Ig的Ig阳性细胞（这些细胞仅对单一轻链类别染色）。此外，对石蜡切片进行IgG和IgM的双重染色已用于表明，同时含有这两类重链的“转换”细胞在反应性淋巴组织中很少见。双重免疫酶技术的潜在应用范围已得到扩展，这体现在该方法适用于冷冻切片（其中抗原反应性比石蜡切片中保存得更好），以及通过制备“单克隆PAP”复合物使其适用于单克隆抗体。
