Schreiber S S, Oratz M, Rothschild M A, Reff F
Cardiovasc Res. 1978 May;12(5):265-8. doi: 10.1093/cvr/12.5.265.
RNA polymerase activity was measured in isolated cardiac nuclei subjected to hydrostatic pressure. After 20 min of pressure, Mn2+ stimulated RNA polymerase II activity was increased. The response to pressure was inhibited by low concentrations of alpha-amanitin (1.1 microgram.cm-3) an inhibitor of polymerase II activity. The data show that pressure applied to isolated nuclei stimulates RNA polymerase II activity, forming mRNA, and suggests that direct application of pressure to cardiac nuclei may be the stimulus which triggers the augmented protein synthesis seen in pressure overload.
在承受静水压力的分离心脏细胞核中测量了RNA聚合酶活性。施加压力20分钟后，锰离子刺激的RNA聚合酶II活性增加。低浓度的α-鹅膏蕈碱（1.1微克·立方厘米）可抑制对压力的反应，α-鹅膏蕈碱是聚合酶II活性的抑制剂。数据表明，对分离细胞核施加压力会刺激RNA聚合酶II活性，形成信使核糖核酸，并表明直接对心脏细胞核施加压力可能是引发压力超负荷时蛋白质合成增加的刺激因素。
