Borzini P, Tedesco F, Greppi N, Rebulla P, Parravicini A, Sirchia G
J Immunol Methods. 1981;44(3):323-32. doi: 10.1016/0022-1759(81)90050-8.
An immunoenzyme assay for detection of platelet antibodies and suitable for routine use is described. Purified rabbit IgG anti-human IgG antibodies are conjugated to beta-galactosidase with meta-maleimidobenzoyl-hydroxysuccinimide ester as a bifunctional reagent and o-nitrophenyl-beta-galactopyranoside as a substrate to evaluate the enzymatic activity of the labeled antiglobulin. The sera of 26 patients suffering from various diseases (acute leukemia, aplastic anemia and systemic lupus erythematosus) and 40 control subjects were assayed with the enzyme-labeled reagent and, for comparison, with an indirect immunofluorescence technique. Half of these patients had never been transfused. Platelet antibodies were detected by both assays in all the transfused patients except one, and in 3 out of 13 non-transfused patients. The sera of all the control subjects were negative. Quantitation of platelet antibodies was obtained by a sensitive antiglobulin absorption technique. A method for standardization of the reagents allowing comparison of results obtained in the same patient at different times and suitable for long-term follow-up studies is also described.
本文描述了一种用于检测血小板抗体且适用于常规检测的免疫酶测定法。以间马来酰亚胺苯甲酰 - 羟基琥珀酰亚胺酯作为双功能试剂，将纯化的兔抗人IgG抗体与β - 半乳糖苷酶偶联，并以邻硝基苯基 - β - 吡喃半乳糖苷作为底物来评估标记抗球蛋白的酶活性。使用酶标记试剂对26例患有各种疾病（急性白血病、再生障碍性贫血和系统性红斑狼疮）的患者以及40例对照者的血清进行检测，并与间接免疫荧光技术进行比较。这些患者中有一半从未接受过输血。除1例输血患者外，两种检测方法在所有输血患者以及13例未输血患者中的3例中均检测到血小板抗体。所有对照者的血清均为阴性。通过敏感的抗球蛋白吸收技术对血小板抗体进行定量。本文还描述了一种试剂标准化方法，该方法可比较同一患者在不同时间获得的结果，适用于长期随访研究。
