一种用于单核细胞增生李斯特菌脂磷壁酸的简单被动血凝抑制试验的开发。
Development of a simple passive haemagglutination-inhibition assay for Listeria monocytogenes lipoteichoic acid.
作者信息
Antonissen A C, van Kessel K P, van Dijk H, Willers J M
出版信息
J Immunol Methods. 1981;44(3):351-7. doi: 10.1016/0022-1759(81)90053-3.
A simple assay for listeria lipoteichoic acid was devised. It is based on the property of this substance to attach spontaneously to erythrocytes. Thus coated rabbit erythrocytes were agglutinated by a rabbit antiserum raised towards dead listeria. Inhibition of this passive agglutination was used as a measure of listeria lipoteichoic acid. Cross-reactivity of lipoteichoic acid from Lactobacillus fermenti in the assay and inhibition by simple sugars suggest that the assay is specific for terminal galactose.
设计了一种用于检测李斯特菌脂磷壁酸的简单测定法。该方法基于这种物质能自发附着于红细胞的特性。因此，用针对死的李斯特菌产生的兔抗血清可使包被的兔红细胞发生凝集。这种被动凝集的抑制作用被用作检测李斯特菌脂磷壁酸的指标。该测定法中发酵乳杆菌的脂磷壁酸存在交叉反应性，且能被单糖抑制，这表明该测定法对末端半乳糖具有特异性。
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