Fietta A, Fortunato A, Dos Santos C, Grassi F, Siccardi A G
Infect Immun. 1981 Sep;33(3):704-9. doi: 10.1128/iai.33.3.704-709.1981.
The bactericidal reactions of normal human serum and of ethylene glycol-bis(beta-aminoethyl ether)-N,N-tetraacetic acid (trisodium salt)-Mg2+-treated human serum differ in a number of aspects. The latter reaction was shown (i) to require the integrity of the alternative pathway of complement, (ii) to display two levels of efficiency depending on the concentration of target bacteria, and (iii) to be modulated by the addition of thermostable serum fractions with inhibitory and stimulatory activities. The inhibitory activities were characterized to a certain extent; they comprised several glycoprotein species of molecular weights between 60,000 and 20,000. The stimulatory activities could be measured only after depletion of the inhibitors by concanavalin A-Sepharose or heat-killed bacteria and could be fractionated into several peaks by gel filtration.
正常人血清以及经乙二醇双（β - 氨基乙醚）-N，N - 四乙酸（三钠盐）-Mg²⁺处理的人血清的杀菌反应在许多方面存在差异。后者的反应表明：（i）需要补体替代途径的完整性；（ii）根据靶细菌的浓度显示出两种效率水平；（iii）可通过添加具有抑制和刺激活性的热稳定血清组分来调节。对抑制活性进行了一定程度的表征；它们包括几种分子量在60,000至20,000之间的糖蛋白。刺激活性只有在通过伴刀豆球蛋白A - 琼脂糖或热灭活细菌耗尽抑制剂后才能测量，并且通过凝胶过滤可分离成几个峰。
