Martinez R J, Carroll S F
Infect Immun. 1980 Jun;28(3):735-45. doi: 10.1128/iai.28.3.735-745.1980.
Several metabolic parameters indicative of Escherichia coli function and integrity were kinetically examined in response to treatment with normal human serum in the presence and absence of functional human lysozyme. Specific inhibition of this enzyme in bacteriolytic and bactericidal reactions was accomplished by using purified rabbit anti-human lysozyme immunoglobulin G. Initiation of the complement-mediated alterations of cytoplasmic membrane integrity, as judged by the leakage of 86Rb from prelabeled cells or the hydrolysis of o-nitrophenyl-beta-D-galactopyranoside by a cryptic strain, was found to be independent of lysozyme action. Furthermore, inhibition of macromolecular synthesis by E. coli in response to serum treatment occurred at the same time regardless of the functional state of lysozyme. Although the rate and extent of bacteriolysis were reduced in the absence of lysozyme, the bactericidal kinetics was unaffected. These results demonstrate that the lethal events associated with the action of antibody and complement on gram-negative bacteria are independent of lysozyme, suggesting an accessory role for this enzyme in immune reactions. A possible temporal sequence of complement-induced effects occurring at the cell surface is presented.
在有和没有功能性人溶菌酶的情况下，对几种指示大肠杆菌功能和完整性的代谢参数进行了动力学检测，以响应正常人血清处理。通过使用纯化的兔抗人溶菌酶免疫球蛋白G，在溶菌和杀菌反应中对该酶进行特异性抑制。通过预标记细胞中86Rb的泄漏或隐性菌株对邻硝基苯基-β-D-吡喃半乳糖苷的水解来判断，补体介导的细胞质膜完整性改变的起始被发现与溶菌酶作用无关。此外，无论溶菌酶的功能状态如何，大肠杆菌对血清处理的大分子合成抑制同时发生。虽然在没有溶菌酶的情况下溶菌的速率和程度降低，但杀菌动力学不受影响。这些结果表明，与抗体和补体对革兰氏阴性菌作用相关的致死事件与溶菌酶无关，表明该酶在免疫反应中起辅助作用。本文提出了补体诱导的细胞表面效应可能的时间顺序。
