Young H, Moyes A
STD Diagnostic Laboratory, Department of Bacteriology, University Medical School, Edinburgh.
Genitourin Med. 1989 Jan;65(1):8-13. doi: 10.1136/sti.65.1.8.
The Phadebact monoclonal gonococcus coagglutination test was evaluated with 1367 (996 anogenital and 371 pharyngeal) neisserial isolates from patients who gave positive diagnostic test results for Neisseria gonorrhoeae. The overall correlation with carbohydrate utilisation was 99.7%. The Phadebact test had a specificity of 100% (286/286) and a sensitivity of 99.7% (1077/1080). The three non-reactive strains were epidemiologically linked and were of a very unusual serovar. Unlike polyclonal antibody based tests, the monoclonal antibody test provides reliable identification of gonococci from anogenital and pharyngeal sites. Because non-reactive strains are rare, however, negative anogenital isolates from heterosexual patients should be tested biochemically. The use of two reagents comprising separate pools of monoclonal antibodies against gonococcal protein IA and IB permitted the identification of an appreciable number of double infections, which would otherwise have been missed. Genital, rectal, and pharyngeal isolates from the same patient should be identified individually.
采用Phadebact单克隆淋球菌协同凝集试验对1367株奈瑟菌分离株（996株来自肛门生殖器部位，371株来自咽部）进行评估，这些分离株均来自淋病奈瑟菌诊断检测结果呈阳性的患者。与碳水化合物利用情况的总体相关性为99.7%。Phadebact试验的特异性为100%（286/286），敏感性为99.7%（1077/1080）。3株无反应性菌株在流行病学上有关联，且属于非常罕见的血清型。与基于多克隆抗体的试验不同，单克隆抗体试验能可靠地鉴定来自肛门生殖器和咽部的淋球菌。然而，由于无反应性菌株很少见，异性恋患者肛门生殖器部位分离出的阴性菌株应进行生化检测。使用两种试剂，分别包含针对淋球菌蛋白IA和IB的不同单克隆抗体池，能够鉴定出相当数量的双重感染，否则这些感染可能会被漏检。来自同一患者的生殖器、直肠和咽部分离株应分别进行鉴定。
