Neurath A R, Strick N
J Virol Methods. 1981 Oct;3(3):155-65. doi: 10.1016/0166-0934(81)90050-1.
A solid-phase enzyme-linked immunoassay using a fluorogenic substrate (4-methylumbelliferyl-beta-D-galactopyranoside) was developed. Antibodies were covalently linked to glutaraldehyde-activated 96-well aminopolystyrene plates. Antigens from test samples were adsorbed to the solid phase and detected using antibodies conjugated with E. coli beta-galactosidase. Glutaraldehyde, N-succinimidyl-3-(2-pyridyldithio)-propionate or N-succinimidyl-6-(4-azido-2-nitrophenylamino)-hexanoate were used as linkers between antibodies and the enzyme. The measurement of fluorescence can be automated for rapid screening of many specimens. The sensitivity limit of the test for HBsAg is about 5-10 pg.
开发了一种使用荧光底物（4-甲基伞形酮基-β-D-吡喃半乳糖苷）的固相酶联免疫测定法。抗体通过共价键连接到戊二醛活化的96孔氨基聚苯乙烯板上。测试样品中的抗原吸附到固相上，并用与大肠杆菌β-半乳糖苷酶偶联的抗体进行检测。戊二醛、N-琥珀酰亚胺基-3-（2-吡啶基二硫代）-丙酸酯或N-琥珀酰亚胺基-6-（4-叠氮基-2-硝基苯氨基）-己酸酯用作抗体与酶之间的连接剂。荧光测量可以自动化，以便快速筛选许多标本。乙肝表面抗原检测的灵敏度极限约为5-10皮克。
