Van Snick J, Uyttenhove C, Van Pel A, Boon T
J Immunol Methods. 1981;46(3):321-6. doi: 10.1016/0022-1759(81)90317-3.
Primed syngeneic or unprimed allogeneic mouse spleen cells were stimulated with azaguanine-resistant P815 tumor cells that were killed by the addition of aminopterin to the stimulation medium. The recovery of lymphocytes and their cytolytic activity and specificity were similar to those obtained after stimulation with irradiated cells. This method conveniently replaces the inactivation of stimulatory cells by irradiation or mitomycin treatment. Moreover, it has the advantage of inactivating not only the stimulatory cells but also the tumor cells that often contaminate the spleens of tumor-bearing animals, provided these animals have been inoculated with azaguanine-resistant tumor cell mutants.
用对氮杂鸟嘌呤有抗性的P815肿瘤细胞刺激经致敏的同基因或未经致敏的异基因小鼠脾细胞，这些肿瘤细胞通过在刺激培养基中添加氨甲蝶呤而被杀死。淋巴细胞的恢复及其细胞溶解活性和特异性与用辐照细胞刺激后获得的结果相似。该方法方便地替代了通过辐照或丝裂霉素处理使刺激细胞失活的方法。此外，它不仅具有使刺激细胞失活的优点，而且还能使经常污染荷瘤动物脾脏的肿瘤细胞失活，前提是这些动物已接种了对氮杂鸟嘌呤有抗性的肿瘤细胞突变体。
