Settergren B, Juto P, Wadell G
J Clin Microbiol. 1987 Jun;25(6):1134-6. doi: 10.1128/jcm.25.6.1134-1136.1987.
A biotin-avidin-amplified indirect immunofluorescence method was used to demonstrate specific serum immunoglobulin M (IgM) antibodies in nephropathia epidemica, the Scandinavian type of hemorrhagic fever with renal syndrome. The antigen in the test was the cross-reacting agent of Korean hemorrhagic fever, Hantaan virus. Sixty-two serum samples from 15 patients with clinically typical nephropathia epidemica were analyzed. Eleven patients had specific IgM in one or more serum samples. The IgM could be demonstrated from day 2 up to day 37, and all patients had detectable specific IgM within 15 days after the onset of disease. In 49 control serum samples, no specific IgM could be detected, indicating a high specificity for the method. The findings demonstrate that the biotin-avidin-amplified immunofluorescence IgM assay is a useful tool in the diagnosis of early nephropathia epidemica disease.
采用生物素-抗生物素蛋白放大间接免疫荧光法检测斯堪的纳维亚型肾综合征出血热即流行性肾病患者血清中的特异性免疫球蛋白M（IgM）抗体。检测中的抗原为朝鲜出血热的交叉反应原——汉坦病毒。对15例临床诊断为典型流行性肾病患者的62份血清样本进行了分析。11例患者的一份或多份血清样本中检测到特异性IgM。发病第2天至第37天均能检测到IgM，且所有患者在发病后15天内均可检测到特异性IgM。49份对照血清样本中未检测到特异性IgM，表明该方法具有较高的特异性。这些结果表明，生物素-抗生物素蛋白放大免疫荧光IgM检测法是早期诊断流行性肾病的一种有用工具。
