Rönnberg L, Kivinen S, Ylikorkala O
Andrologia. 1981 Sep-Oct;13(5):406-11. doi: 10.1111/j.1439-0272.1981.tb00072.x.
To elucidate the mechanism of clomiphene action in the treatment of male infertility, we studied the effect of three-month clomiphene treatment (50 mg daily) on the radioimmunoassayable concentrations of gonadotropins (FSH, LH), prolactin (PRL), estradiol (E2), and testosterone (T) in seminal plasma and peripheral serum in eleven normogonadotropic subfertile men. Clomiphene therapy increased (p less than 0.01) the levels of FSH and LH in seminal plasma, but to a lesser extent (p less than 0.01) than in peripheral serum. PRL did not change is seminal plasma or peripheral serum. The seminal plasma and peripheral serum T concentrations doubled following clomiphene intake without any demonstrable correlation with the sperm count. The concentrations of E2 increased about ten times (p less than 0.005) in seminal plasma, a significantly greater (p less than 0.05) increase than that in the serum. There was a significant correlation (r = 0.804, p less than 0.01) between the sperm counts and seminal plasma E2 levels following clomiphene treatment. These results suggest that Sertoli cell activation may be a key event in clomiphene action in the treatment of male infertility.
为阐明克罗米芬治疗男性不育症的作用机制，我们研究了为期三个月的克罗米芬治疗（每日50毫克）对11名正常促性腺激素水平的亚生育男性精液血浆和外周血清中促性腺激素（FSH、LH）、催乳素（PRL）、雌二醇（E2）和睾酮（T）的放射免疫分析可测浓度的影响。克罗米芬治疗使精液血浆中FSH和LH水平升高（p<0.01），但升高程度低于外周血清（p<0.01）。PRL在精液血浆或外周血清中未发生变化。服用克罗米芬后，精液血浆和外周血清中的T浓度翻倍，且与精子计数无明显相关性。精液血浆中E2浓度增加约10倍（p<0.005），增幅显著大于血清（p<0.05）。克罗米芬治疗后精子计数与精液血浆E2水平之间存在显著相关性（r = 0.804，p<0.01）。这些结果表明，支持细胞激活可能是克罗米芬治疗男性不育症作用的关键事件。
