Rönnberg L, Vihko P, Sajanti E, Vihko R
Int J Androl. 1981 Jun;4(3):372-8. doi: 10.1111/j.1365-2605.1981.tb00721.x.
Clomiphene citrate was administered as a 50 mg oral daily dose to 44 normogonadotrophic (serum FSH 2-10 mIU/ml) subfertile men for 3 months. The treatment resulted in significant increases in FSH and LH concentrations, whereas prolactin remained unchanged. Serum testosterone and oestradiol both increased highly significantly. The increased testosterone levels suggest that the elevated LH levels had not led to "down regulation" of Leydig cell LH/hCG receptors, neither had the greatly increased estradiol led to depletion of these receptors. This is suggested to be a result of the blocking of testicular oestradiol receptors by the estrogen antagonist, clomiphene. Sperm count increased highly unchanged. The spermatic fluid concentrations of zinc and magnesium ions were also increased, whereas fructose remained unchanged. The katalytic activity of acid phosphatase in spermatic fluid increased highly significantly, whereas the concentration of the main prostate-specific acid phosphatase, as measured by a specific radioimmunological method, remained unchanged. Therefore, the increased Zn and Mg ion concentrations may be responsible for activation of acid phosphatase (s) in semen, or the treatment led to increased secretion of other prostatic acid phosphatase(s) than the main enzyme. However, it is clear that the secretion of the main prostatic acid phosphatase into semen is under different control than that of Zn++ and Mg++.
对44名促性腺激素正常（血清促卵泡激素2 - 10 mIU/ml）的不育男性，每天口服50毫克枸橼酸氯米芬，持续3个月。治疗导致促卵泡激素和促黄体生成素浓度显著升高，而催乳素保持不变。血清睾酮和雌二醇均极显著升高。睾酮水平升高表明，促黄体生成素水平升高并未导致睾丸间质细胞促黄体生成素/人绒毛膜促性腺激素受体的“下调”，雌二醇大幅升高也未导致这些受体的耗竭。这被认为是雌激素拮抗剂氯米芬阻断睾丸雌二醇受体的结果。精子数量极显著增加。精液中锌和镁离子的浓度也升高，而果糖保持不变。精液中酸性磷酸酶的催化活性极显著增加，而通过特定放射免疫方法测定的主要前列腺特异性酸性磷酸酶的浓度保持不变。因此，锌和镁离子浓度升高可能是精液中酸性磷酸酶激活的原因，或者该治疗导致了除主要酶之外的其他前列腺酸性磷酸酶分泌增加。然而，很明显，主要前列腺酸性磷酸酶分泌到精液中的调控机制与锌离子和镁离子的不同。
