从蓝藻弗里茨绿球藻中分离光系统1和光系统2时对乙二胺四乙酸（EDTA）的需求。
A requirement for EDTA in the separation of Photosystems 1 and 2 from the cyanobacterium Chlorogloea fritschii.
作者信息
Evans E H, Pullin C A
出版信息
Biochem J. 1981 May 15;196(2):489-93. doi: 10.1042/bj1960489.
Abstract
Fractions enriched in Photosystem 1 or Photosystem 2 activity have been isolated from the cyanobacterium Chlorogloea fritschii after extraction of the membranes with digitonin and Triton X-100. Separation of the extract into the two components was achieved by using a Sepharose 6B column, calibration of which gave Kd values of 0.3 for the Photosystem 1 fraction and 0.53 for Photosystem 2. These values corresponded to molecular weights of approx. 500000 and 90000 respectively. The Photosystem 1 particle was shown to aggregate on storage and EDTA was shown to be necessary to separate the Photosystem 1 and 2 fractions.
摘要
在用洋地黄皂苷和 Triton X - 100 提取膜后，从蓝藻弗里茨绿球藻中分离出了富含光系统 1 或光系统 2 活性的组分。通过使用 Sepharose 6B 柱将提取物分离为两个组分，该柱的校准表明光系统 1 组分的 Kd 值为 0.3，光系统 2 的 Kd 值为 0.53。这些值分别对应于约 500000 和 90000 的分子量。结果表明，光系统 1 颗粒在储存时会聚集，并且 EDTA 对于分离光系统 1 和 2 组分是必需的。
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