Ludden P W
Biochem J. 1981 Aug 1;197(2):503-5. doi: 10.1042/bj1970503.
Borate and aminophenylboronic acid were tested as inhibitors of activation of inactive dinitrogenase reductase from Rhodospirillum rubrum. Inhibition was specific for activation because activity of the active form of the enzyme was not inhibited. Inhibition showed the pH-dependence expected if borate inhibits by binding to cis-hydroxy groups of the modifying group found on the inactive enzyme.
硼酸盐和氨基苯硼酸作为来自红螺菌的无活性固氮酶还原酶激活抑制剂进行了测试。抑制作用对激活具有特异性,因为该酶活性形式的活性未受抑制。如果硼酸盐通过与无活性酶上修饰基团的顺式羟基结合来抑制,那么抑制作用表现出预期的pH依赖性。