从深红红螺菌中激活固氮酶还原酶过程中腺嘌呤样分子的去除。
Removal of an adenine-like molecule during activation of dinitrogenase reductase from Rhodospirillum rubrum.
作者信息
Ludden P W, Burris R H
出版信息
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6201-5. doi: 10.1073/pnas.76.12.6201.
Abstract
During the activation of the inactive dinitrogenase reductase from Rhodospirillum rubrum, an adenine-like molecules is lost and phosphate is found on both active and inactive forms of the protein. ATP and divalent metals are required for activation of the reduced protein, but ATP is not required for activation of phenazine methosulfate-oxidized dinitrogenase reductase. Snake venom diesterase and spleen diesterase have no effect on the inactive protein; alkaline phosphatase removes phosphate from the activated protein but not from the inactive protein. ATP binds to both active and inactive forms of the protein.
摘要
在激活来自红螺菌的无活性二氮酶还原酶的过程中，一种类似腺嘌呤的分子会丢失，并且在该蛋白质的活性和非活性形式中都发现了磷酸盐。还原型蛋白质的激活需要ATP和二价金属，但吩嗪硫酸甲酯氧化的二氮酶还原酶的激活不需要ATP。蛇毒二酯酶和脾二酯酶对无活性蛋白质没有影响；碱性磷酸酶可从激活的蛋白质中去除磷酸盐，但不能从无活性蛋白质中去除。ATP与该蛋白质的活性和非活性形式都结合。
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本文引用的文献
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Substrate and light dependent fixation of molecular nitrogen in Rhodospirillum rubrum.深红红螺菌中分子氮的底物及光依赖固定作用
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An effect of magnesium adenosine 5'-triphosphate on the structure of azoferredoxin from Clostridium pasteurianum.腺苷-5'-三磷酸镁对巴氏芽孢梭菌偶氮铁氧化还原蛋白结构的影响。
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Fluorometric determination of adenine and its derivatives by reaction with glyoxal hydrate trimer.通过与水合乙二醛三聚体反应荧光法测定腺嘌呤及其衍生物
Anal Biochem. 1972 Mar;46(1):123-8. doi: 10.1016/0003-2697(72)90403-4.
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Activating factor for the iron protein of nitrogenase from Rhodospirillum rubrum.深红红螺菌固氮酶铁蛋白的激活因子。
Science. 1976 Oct 22;194(4263):424-6. doi: 10.1126/science.824729.
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Regulation of nitrogenase synthesis in intact cells of Rhodospirillum rubrum: inactivation of nitrogen fixation by ammonia, L-glutamine and L-asparagine.深红红螺菌完整细胞中固氮酶合成的调控：氨、L-谷氨酰胺和L-天冬酰胺对固氮作用的抑制
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