Landi F L, Borrello S, Serri F
Boll Soc Ital Biol Sper. 1981 Nov 30;57(22):2298-302.
The object of the research is to classify the nuclear proteins of the cellular nucleus of some types of human skin tumours (melanotic and amelanotic melanomas, squamous epitheliomas). Nuclear proteins were extracted from the nuclei with a saline buffer. Nuclear proteins were extracted from a melanoma step by step. After prolonged centrifugation to sediment the DNA and the large ribonucleoprotein complexes, the nuclear proteins were chromatographed on columns of Biorex 70+ to remove proteins were chromatographed on columns of Biorex 70+ to remove the histones. Nuclear proteins from a squamous epithelioma were injected into a rabbit to induce specific antibodies.
该研究的目的是对某些类型人类皮肤肿瘤（黑素性和无黑素性黑色素瘤、鳞状上皮瘤）细胞核中的核蛋白进行分类。用盐缓冲液从细胞核中提取核蛋白。从黑色素瘤中逐步提取核蛋白。经过长时间离心使DNA和大的核糖核蛋白复合物沉淀后，将核蛋白在Biorex 70+柱上进行色谱分离以去除组蛋白。将来自鳞状上皮瘤的核蛋白注射到兔子体内以诱导产生特异性抗体。
