Solid phase enzyme immunoassay of tobramycin.

We have developed a competitive enzyme immunoassay (EIA) using co-polymer of styrene and maleate as the solid phase and glutaraldehyde for binding of the antibody to the solid phase. This assay showed a striking efficacy in its use for the assay of tobramycin (TOB). Furthermore, the pharmacokinetics of TOB were studied in six healthy male volunteers after the administration of 60- or 90-mg doses intramuscularly.