Tsay Y G, Palmer R J
Clin Chim Acta. 1981 Jan 22;109(2):151-7. doi: 10.1016/0009-8981(81)90329-6.
A solid-phase fluoroimmunoassay has been developed for the determination of tobramycin in serum. The principle of this method is based on direct competition of a fluorescein-labeled and unlabeled hapten for a fixed amount of specific antibody immobilized on a dipstick sampler. The bound and free labeled hapten are separated simply by removing the sampler from the reaction mixture. The fluorescence signal from the bound fluorescein-labeled hapten is measured with a FIAX fluorometer. The fluorescein-labeled tobramycin, which is easily prepared, has excellent shelf life. The assay is fast and simple, involves only one pipetting step, and avoids the use of radiochemicals. Within-assay coefficients of variation are less than 10%. The assay correlates well with radioimmunoassay (r = 0.97) and radioenzymatic assay (r = 0.96).
已开发出一种用于测定血清中妥布霉素的固相荧光免疫测定法。该方法的原理基于荧光素标记和未标记的半抗原对固定量固定在试纸条采样器上的特异性抗体的直接竞争。通过从反应混合物中取出采样器，即可简单地分离结合的和游离的标记半抗原。用FIAX荧光计测量结合的荧光素标记半抗原的荧光信号。易于制备的荧光素标记妥布霉素具有出色的保质期。该测定法快速简便，仅涉及一个移液步骤，且避免使用放射化学物质。批内变异系数小于10%。该测定法与放射免疫测定法（r = 0.97）和放射酶测定法（r = 0.96）相关性良好。
