Gadner H, Odenwald E, Riehm H, Hitzig W H
Eur J Pediatr. 1978 Aug 17;129(1):37-45. doi: 10.1007/BF00441372.
For several reasons, electronic platelet counts have not been generally adopted in paediatric routine laboratories. A method of platelet counting in capillary blood samples using an electronic counter is described. This is based on brief centrifugation of the blood sample after the addition of a diluent containing heparin/dextran and with a density of 1.050. The thrombocytes are uniformly suspended in the supernatant after 5 min of centrifugation at 250 g, so that the counting procedure in the electric unit can follow immediately. This simplified, dependable and reproducible method is also suitable for venous blood, and as a result of several years of successful trial, it can be recommended for routine use, especially in clinics with paediatric patients.
由于多种原因，电子血小板计数尚未在儿科常规实验室中普遍采用。本文描述了一种使用电子计数器对毛细血管血样进行血小板计数的方法。该方法基于在加入含有肝素/右旋糖酐且密度为1.050的稀释剂后，对血样进行短暂离心。在250g离心5分钟后，血小板均匀悬浮在上清液中，以便立即在电子装置中进行计数程序。这种简化、可靠且可重复的方法也适用于静脉血，经过数年的成功试验，可推荐用于常规使用，尤其是在有儿科患者的诊所。
