Skoglund M L, Nies A S, Gerber J G
J Pharmacol Exp Ther. 1982 Feb;220(2):371-4.
Isolated canine parietal cells were used to evaluate inhibition of acid secretion by both endogenously synthesized and exogenously added prostaglandins (PGs). Uptake of [14C]aminopyrine by the parietal cells was used as an index of acid secretion. The effect of increasing endogenous PG synthesis was determined by incubating arachidonic acid with histamine-stimulated parietal cells. Arachidonic acid (10(-6) and 10(-5) M) was a potent inhibitor of histamine-stimulated acid secretion. This effect of arachidonic acid was totally abolished when the cells were pretreated with 100 microM indomethacin. The effect of exogenous, preformed PGs on histamine-stimulated acid secretion was evaluated by adding PGE2, PGI2, PGF2 alpha or PGD2 to parietal cells. PGE2 was the most potent inhibitor of acid secretion, with a 50% inhibition of maximal acid secretion (ID50) at 7.5 X 10(-8) M. PGI2 and PGF2 alpha also inhibited acid secretion, but at higher concentrations. The ID50 for PGI2 and PGF2 alpha was 10(-5) M. PGD2 was inactive at inhibiting acid secretion. We conclude that both endogenous and exogenous PGs inhibit acid secretion directly at the parietal cells and that PGE2 is most likely the PG produced locally to modulate parietal cell acid secretion.
分离的犬壁细胞用于评估内源性合成和外源性添加的前列腺素（PGs）对胃酸分泌的抑制作用。壁细胞对[14C]氨基比林的摄取被用作胃酸分泌的指标。通过将花生四烯酸与组胺刺激的壁细胞孵育来确定增加内源性PG合成的效果。花生四烯酸（10^(-6)和10^(-5) M）是组胺刺激的胃酸分泌的有效抑制剂。当细胞用100 microM消炎痛预处理时，花生四烯酸的这种作用完全被消除。通过向壁细胞中添加PGE2、PGI2、PGF2α或PGD2来评估外源性预制PGs对组胺刺激的胃酸分泌的影响。PGE2是胃酸分泌最有效的抑制剂，在7.5×10^(-8) M时对最大胃酸分泌有50%的抑制作用（半数抑制浓度ID50）。PGI2和PGF2α也抑制胃酸分泌，但浓度较高。PGI2和PGF2α的ID50为10^(-5) M。PGD2在抑制胃酸分泌方面无活性。我们得出结论，内源性和外源性PGs均直接在壁细胞处抑制胃酸分泌，并且PGE2很可能是局部产生的调节壁细胞胃酸分泌的PG。
