Asselineau C, Clavel S, Clément F, Daffé M, David H, Lanéelle M A, Promé J C
Ann Microbiol (Paris). 1981 Jan-Feb;132A(1):19-30.
Mycobacterium leprae (obtained from experimentally infected armadillo) was submitted to saponification. The liposoluble part was methylated and fractionated by chromatographic methods. Each fraction was studied by gas-liquid chromatography. Cholesterol (from the infected host) and the main fatty acids were identified. Mycolic acids were isolated, and their structures determined, using mass spectrometry. These structures are useful to make a comparison of M. leprae with some other mycobacteria. Some of these comparisons are discussed here. The absence-or, at least, the very low level-of tuberculostearate suggests comparative studies of M. leprae and M. gordonae.
将从实验感染的犰狳中获取的麻风分枝杆菌进行皂化处理。将脂溶性部分进行甲基化,并通过色谱方法进行分离。对每个馏分进行气液色谱分析。鉴定出(来自受感染宿主的)胆固醇和主要脂肪酸。分离出分枝菌酸,并使用质谱法确定其结构。这些结构有助于将麻风分枝杆菌与其他一些分枝杆菌进行比较。本文讨论了其中的一些比较。结核硬脂酸的缺失——或者至少是极低水平——表明需要对麻风分枝杆菌和戈登分枝杆菌进行比较研究。