Mather E L, Perry R P
Nucleic Acids Res. 1981 Dec 21;9(24):6855-67. doi: 10.1093/nar/9.24.6855.
The relative transcriptional activity of rearranged and unrearranged (germline) VK genes in secreting plasmacytoma cells was assessed by two independent methods. Measurements of V sequence abundance by hybridization kinetic (Rot) analysis indicated that the steady state content of transcripts from a rearranged VK gene is at least 16,000-fold greater than that from an unrearranged VK gene. Direct measurements of transcriptional activity in isolated nuclei indicated that this difference is due, in large part to a difference in transcription rate. Since the primary sequences of V genes and their 5' flanking regions are not altered during rearrangement, these results suggest that VK gene transcription might be controlled by elements on the 3' side of the VK genes or at the CK locus, perhaps via an influence on chromatin structure.
通过两种独立方法评估了分泌性浆细胞瘤细胞中重排和未重排（种系）VK基因的相对转录活性。通过杂交动力学（Rot）分析对V序列丰度进行测量，结果表明重排的VK基因转录本的稳态含量比未重排的VK基因至少高16000倍。对分离细胞核中转录活性的直接测量表明，这种差异在很大程度上是由于转录速率的差异。由于V基因及其5'侧翼区域的一级序列在重排过程中未发生改变，这些结果表明VK基因转录可能受VK基因3'端或CK基因座上的元件控制，也许是通过对染色质结构的影响来实现的。
