Landfear S M, McMahon-Pratt D, Wirth D F
Mol Cell Biol. 1983 Jun;3(6):1070-6. doi: 10.1128/mcb.3.6.1070-1076.1983.
The arrangement of developmentally regulated alpha- and beta-tubulin genes has been studied in the parasitic protozoan Leishmania enriettii by using Southern blot hybridization analysis. The alpha-tubulin genes occur in a tandem repeat whose monomeric unit may be represented by a 2-kilobase PstI fragment. Similarly, the beta-tubulin genes probably occur in a separate tandem repeat consisting of approximately 4-kilobase units unlinked to the alpha-tubulin repeats.
通过Southern印迹杂交分析,研究了寄生原生动物恩氏利什曼原虫中发育调控的α-和β-微管蛋白基因的排列。α-微管蛋白基因以串联重复的形式出现,其单体单元可能由一个2千碱基的PstI片段代表。同样,β-微管蛋白基因可能以单独的串联重复形式出现,由大约4千碱基的单元组成,与α-微管蛋白重复序列不相连。